Nelson:Protocols: Difference between revisions
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== Protocols == | == Protocols == | ||
Rubidium Chloride competent cells [[Wiese_Lab:Competent_Cell_Prep]]<br> | |||
Calcium Chloride competent cells [[Kafatos:competent cells]]<br> | |||
Qiagen Miniprep [[Minipreps]]<br> | |||
[[DNA_Precipitation]]<br> | |||
[[DNA_ligation]]<br> | |||
Quickchange Mutagenesis [[Knight:Site-directed_mutagenesis/Single_site]]<br> | |||
[http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/d3764pis.Par.0001.File.tmp/d3764pis.pdf preparing DEAE-cellulose ] | |||
Revision as of 09:30, 3 April 2009
Recipes
LB
M9 medium/minimal
TBE
SOB
SOC
10 X SDS-PAGE running buffer
50 X TAE buffer
Phosphate_buffer
Protocols
Rubidium Chloride competent cells Wiese_Lab:Competent_Cell_Prep
Calcium Chloride competent cells Kafatos:competent cells
Qiagen Miniprep Minipreps
DNA_Precipitation
DNA_ligation
Quickchange Mutagenesis Knight:Site-directed_mutagenesis/Single_site
preparing DEAE-cellulose
