NanoBio: Stock Solutions

From OpenWetWare
Revision as of 12:50, 25 April 2013 by CarolineAjo-Franklin (talk | contribs) (→‎Stock Solutions)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigationJump to search

Stock Solutions

  • aminolevulinic acid (δ-ALA), 1 M stock
    • Sigma catalog #A7793-1G, MW 167.59, stored at -20°C
    • dissolve 0.167 g in 0.9 mL water
    • adjust final volume to 1 mL
    • store stock solution at -20°C
  • equine cytochrome c, 1 mM stock
    • VWR catalog #, CalBiochem catalog #250600; MW 12,270; stored at -20°C
    • dissolve 6.13 mg in 0.5 mL water
    • portion into 50 uL aliquots,
    • store at -20°C for up to 6 months
  • HEPES pH 7.5, 1 M stock
  • isopropyl β-D-1-thiogalactopyranoside(IPTG), 1 M stock
  • sodium dithionite (Na2S2O4), 1 M stock
    • Sigma catalog # 157953-100G, MW 174.1, original bottle and aliquoted stored at room temperature in a dessicated, sealed container
    • dissolve 0.174 g in 1 mL water
  • CAjoF 15:48, 25 April 2013 (EDT):

return to Protocols