NanoBio:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 12: | Line 12: | ||
*[[NanoBio: Oligonucleotide Inserts | Oligonucleotide Inserts]] | *[[NanoBio: Oligonucleotide Inserts | Oligonucleotide Inserts]] | ||
*[[NanoBio: Overlapping Oligonucleotide Inserts | Overlapping Oligonucleotide Inserts]] | *[[NanoBio: Overlapping Oligonucleotide Inserts | Overlapping Oligonucleotide Inserts]] | ||
*[[ | *[[NanoBio: Site-Directed Mutagenesis | Site-Directed Mutagenesis]] | ||
*[[Silver: Restriction Digest | Restriction Digest]] | *[[Silver: Restriction Digest | Restriction Digest]] | ||
*[[Silver: Ligation | Ligation]] | *[[Silver: Ligation | Ligation]] | ||
Revision as of 11:36, 26 January 2008
Nano Synth Bio
General Molecular Biology
Current Protocols in Molecular Biology
Sub-cloning using Biobricks or Biofusion Vectors
- Note: Many of these are protocols that I used and/or developed as a postdoctoral fellow in Pam Silver's lab.
- Strategy for Making Parts
- Designing Parts or Genes
- Commonly Used Plasmids
- Commonly Used Primers
- PCR
- Oligonucleotide Inserts
- Overlapping Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- Ligation
- Quick & Dirty Digest & Ligation
- Bacterial Transformation
- Plasmid Verification
Genomic Integration into S. cerevisiae
Lipid Bilayers
Care of Synechocystis sp 6803
Primers
- Biobricks & Biofusion Sub-cloning
- Synechocystis sp. 6803
- Shewanella oneidensis MR-1
- Guidelines on Talking or Writing about your project
- Microbial Respiration
