Nachury:Protocols: Difference between revisions
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==Protocols== | ==Protocols== | ||
<center><font color="red">Under construction</font></center> | <center><font color="red">Under construction</font></center> | ||
===Cell Lines=== | |||
:*[[Nachury:Retrovirus_Infection|Establishing stable lines with retrovirus]] | |||
:*[[Nachury:FACS_Cloning|Cloning fluorescent cells by FACS]] | |||
:*[[Nachury:Cell_expansion|Growing and harvesting cells in large amounts]] | |||
===Extractology=== | ===Extractology=== | ||
:*[[Nachury:Nuclear_Extract|Hypotonic/N<sub>2</sub> cavitation lysis]] | :*[[Nachury:Nuclear_Extract|Hypotonic/N<sub>2</sub> cavitation lysis]] | ||
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:*[[Nachury:Retinal_Extract|Retinal extract]] | :*[[Nachury:Retinal_Extract|Retinal extract]] | ||
:*[[Nachury:Retinal_Detergent_Extract|Retinal detergent extract]] | :*[[Nachury:Retinal_Detergent_Extract|Retinal detergent extract]] | ||
===Protein Biochemistry=== | ===Protein Biochemistry=== | ||
:*[[Nachury:TAP|TAP purification of protein complexes from mammalian cells]] | |||
:*[[Nachury:silver|Silver staining]] | :*[[Nachury:silver|Silver staining]] | ||
:*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]] | :*[[Nachury:MeOH_CHCl3|Methanol-Chloroform precipitation of proteins]] | ||
Revision as of 19:55, 9 January 2008
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ProtocolsCell LinesExtractologyProtein BiochemistryImmunofluorescenceLipidsDNA |
