NEB buffers

Composition of NEBuffers (1x):

NEBuffer 1 (yellow)

10 mM Bis Tris Propane-HCl, 10 mM MgCl2, 1 mM DTT (pH 7.0 at 25°C).

NEBuffer 2 (blue)

10 mM Tris-HCl, 10 mM MgCl2, 50 mM NaCl, 1 mM DTT (pH 7.9 at 25°C).

NEBuffer 3 (red)

50 mM Tris-HCl, 10 mM MgCl2, 100 mM NaCl, 1 mM DTT (pH 7.9 at 25°C).

NEBuffer 4 (green)

20 mM Tris-acetate, 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT (pH 7.9 at 25°C).

T4 DNA Ligase Buffer

50 mM Tris-HCl, 10 mM MgCl2, 1 mM ATP, 10 mM Dithiothreitol (pH 7.5 at 25°C).

NEBuffer EcoRI

100 mM Tris-HCl, 50 mM NaCl, 10 mM MgCl2, 0.025 % Triton X-100 (pH 7.5 at 25°C).

Source: NEB and