NEB buffers

(Difference between revisions)

Current revision

Composition of NEBuffers (1x):

NEBuffer 1 (yellow): 10 mM Bis Tris Propane-HCl, 10 mM MgCl2, 1 mM DTT (pH 7.0 at 25°C).

NEBuffer 2 (blue): 10 mM Tris-HCl, 10 mM MgCl2, 50 mM NaCl, 1 mM DTT (pH 7.9 at 25°C).

NEBuffer 3 (red): 50 mM Tris-HCl, 10 mM MgCl2, 100 mM NaCl, 1 mM DTT (pH 7.9 at 25°C).

NEBuffer 4 (green): 20 mM Tris-acetate, 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT (pH 7.9 at 25°C).

T4 DNA Ligase Buffer: 50 mM Tris-HCl, 10 mM MgCl2, 1 mM ATP, 10 mM Dithiothreitol (pH 7.5 at 25°C).

NEBuffer EcoRI: 100 mM Tris-HCl, 50 mM NaCl, 10 mM MgCl2, 0.025 % Triton X-100 (pH 7.5 at 25°C).

Source: NEB and

@@ Line 9: / Line 9: @@
 ; NEBuffer 4 (green) : 20 mM Tris-acetate, 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT (pH 7.9 at 25&deg;C).
-; T4 DNA Ligase Buffer : 50 mM Tris-HCl, 10 mM MgCl2, 1 mM ATP, 10 mM Dithiothreitol, (pH 7.5 at 25&deg;C).
+; T4 DNA Ligase Buffer : 50 mM Tris-HCl, 10 mM MgCl2, 1 mM ATP, 10 mM Dithiothreitol (pH 7.5 at 25&deg;C).
+; NEBuffer EcoRI : 100 mM Tris-HCl, 50 mM NaCl, 10 mM MgCl2, 0.025 % Triton X-100 (pH 7.5 at 25&deg;C).
 Source: [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/buffer_legend.asp? NEB]
+[[Category:Material]] and [[Category:Buffers]]