Muratore:Protocols/Digest/SapI

From OpenWetWare
Revision as of 11:02, 26 May 2011 by Kathryn Muratore (talk | contribs) (New page: {|border="1" |+ '''SapI digest protocol''' |- !Tube !!sterile H<sub>2</sub>O !!10X NEB4 !!DNA !!SapI (2 kU/mL) |- !plasmid |30 μL ||5 μL ||10 μL of NheI digest ||5μL |- !insert |30 μL...)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigationJump to search
SapI digest protocol
Tube sterile H2O 10X NEB4 DNA SapI (2 kU/mL)
plasmid 30 μL 5 μL 10 μL of NheI digest 5μL
insert 30 μL 5 μL 10 μL of NheI digest 5μL
  1. 2h @ 37°C
  2. spin-column purify
Retrieved from "https://openwetware.org/mediawiki/index.php?title=Muratore:Protocols/Digest/SapI&oldid=511477"