Muratore:Protocols/Digest/NheI

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Revision as of 16:53, 26 May 2011 by Kathryn Muratore (Talk | contribs)
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NheI digest protocol
Tube sterile H2O 10X NEB2 100X BSA DNA NheI (10 kU/mL)
plasmid 34.5 μL 5 μL 0.5 μL 5 μL of 100-500 μg/mL 5μL
insert 24.5 μL 5 μL 0.5 μL 15 μL from purified PCR reaction 5μL
  1. 2h @ 37°C
  2. 20' @ 65°C (heat kill)
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