Muratore:Materials/Media/LB: Difference between revisions
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(Undo revision 529906 by Kathryn Muratore (Talk)) |
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Bottles of 50 mL [[LB]] | ==Bottles of 50 mL [[LB]]== | ||
*1 g Bacto-tryptone | *1 g Bacto-tryptone | ||
*0.5 g Yeast extract | *0.5 g Yeast extract | ||
*1 g NaCl | *1 g NaCl | ||
#add water to 50 mL (use graduated cylinder) and transfer to 200 mL Wheaton bottles | |||
#autoclave with caps loose | |||
#store long term at 4°C or for immediate use at room T | |||
====History:==== | |||
5/24/2011 by [[User:Kathryn Muratore|Kathryn Muratore]] | |||
[[Category:Material]] | [[Category:Material]] | ||
[[Category:Media]] | [[Category:Media]] | ||
Latest revision as of 12:55, 16 August 2011
Bottles of 50 mL LB
- 1 g Bacto-tryptone
- 0.5 g Yeast extract
- 1 g NaCl
- add water to 50 mL (use graduated cylinder) and transfer to 200 mL Wheaton bottles
- autoclave with caps loose
- store long term at 4°C or for immediate use at room T
History:
5/24/2011 by Kathryn Muratore
