Mounting medium

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A '''Mounting medium''' is a liquid, polymer, resin, etc, used to mount microscopical specimens before examination. Its optical attributes (refractive index, transparency) and physical/chemical properties (viscosity, stability, preservation of the specimen) are crucial. Mounting media for fluorescent microscopy often contain anti-fading agents to lengthen the life of fluorophores by quenching free radicals generated during illumination of the specimen.
=== See also on OWW ===
=== See also on OWW ===
* [[Immunofluorescence Microscopy (Pf, fixed)]]
* [[Immunofluorescence Microscopy (Pf, fixed)]]
* [[Griffin:Immunofluorescence Cell Staining]]
* [[Griffin:Immunofluorescence Cell Staining]]
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=== Related web resources ===
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* [[Image:3stars.png]] [http://www.gonda.ucla.edu/bri_core/mounting.htm Mounting medium page by UCLA Brain Institute Imaging Facility]
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* [[Image:3stars.png]] [http://www.ihcworld.com/_protocols/histology/aqueous_mounting_medium.htm Page with recipes and evaluation of 5 different mounting media by JA Kiernan at IHCWorld]
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[[Category:Protocol]]
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[[Category:Chemical]]
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[[Category:Microscopy]]

Current revision

A Mounting medium is a liquid, polymer, resin, etc, used to mount microscopical specimens before examination. Its optical attributes (refractive index, transparency) and physical/chemical properties (viscosity, stability, preservation of the specimen) are crucial. Mounting media for fluorescent microscopy often contain anti-fading agents to lengthen the life of fluorophores by quenching free radicals generated during illumination of the specimen.

See also on OWW

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