Moore:Protocols

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(E. coli Strain Construction)
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[[%27Round-the-horn_site-directed_mutagenesis|'Round-the-Horn Mutagenesis]]<br>
[[%27Round-the-horn_site-directed_mutagenesis|'Round-the-Horn Mutagenesis]]<br>
[[Sauer:Stitching_Genes_by_PCR|Gene Stitching]]<br>
[[Sauer:Stitching_Genes_by_PCR|Gene Stitching]]<br>
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[[Library_Generation|Making Genomic Libraries]]<br>
<br>
<br>
===Protein Purification===
===Protein Purification===
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[[Sauer:bis-Tris_SDS-PAGE%2C_the_very_best|SDS PAGE]]<br>
[[Sauer:bis-Tris_SDS-PAGE%2C_the_very_best|SDS PAGE]]<br>
[[Sauer:Western_blot|Western Blot]]<br>
[[Sauer:Western_blot|Western Blot]]<br>
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<br>
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===Enymatic Assays===
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<br>
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[[Beta-Galactosidase_Assay_%28A_better_Miller%29|Miller/β-Galactosidase Assay]]<br>
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<br>
<br>
===Translation and Ribosomes===
===Translation and Ribosomes===

Revision as of 17:55, 2 July 2008


Contents

E. coli Strain Construction


Working With E. coli
Transducing Genes with P1 Phage

Cloning and Molecular Biology


Figuring Out How Much Nucleic Acid You Have
'Round-the-Horn Mutagenesis
Gene Stitching
Making Genomic Libraries

Protein Purification


Getting the Cells open
E. coli ClpX
E. coli ClpP
E. coli SspB

Protein Analysis


Silver Staining
SDS PAGE
Western Blot

Enymatic Assays


Miller/β-Galactosidase Assay


Translation and Ribosomes


E. coli Ribosome Purification
Preparing Translation Extracts
Poly-U Translation
mRNA Translation

RNA Purification and Analysis


Getting RNA From E. coli
Primer Extension

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