Moore:Chemiluminescent

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(New page: ==Chemiluminescent HRP Detection for Western Blotting== ===Protocol Background=== ===Recipes of Stocks=== ===Recipes of Working Solutions=== ===Procedure=== ===Comments===)
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===Protocol Background===
===Protocol Background===
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This protocol is presented as a translation from a sheet of paper I received from a colleague.  It describes a luminol-based chemiluminescent protocol for detecting horseradish peroxidase-conjugated antibodies during Western immunodetection.  This protocol serves to replaces the commercially available ECL and ECL PLus technologies sold and licensed by GE Healthcare.  The Protocol I obtained is titled "ECL SOUTHERN / WESTERNS DETECTION REAGENTS" with the footnote "adapted from St. Geme and Miller lab protocols 1/4/01 by her highness Ms. J. Sexton".  I don't know who these folks are, but thank you very much, you save us a lot of money.
===Recipes of Stocks===
===Recipes of Stocks===

Revision as of 10:43, 12 November 2008

Contents

Chemiluminescent HRP Detection for Western Blotting

Protocol Background

This protocol is presented as a translation from a sheet of paper I received from a colleague. It describes a luminol-based chemiluminescent protocol for detecting horseradish peroxidase-conjugated antibodies during Western immunodetection. This protocol serves to replaces the commercially available ECL and ECL PLus technologies sold and licensed by GE Healthcare. The Protocol I obtained is titled "ECL SOUTHERN / WESTERNS DETECTION REAGENTS" with the footnote "adapted from St. Geme and Miller lab protocols 1/4/01 by her highness Ms. J. Sexton". I don't know who these folks are, but thank you very much, you save us a lot of money.

Recipes of Stocks

Recipes of Working Solutions

Procedure

Comments

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