Miniprep/GET buffer - Revision history

http://openwetware.org/index.php?title=Miniprep/GET_buffer&feed=atom&action=history Miniprep/GET buffer - Revision history 2013-05-20T00:59:11Z Revision history for this page on the wiki MediaWiki 1.13.2 http://openwetware.org/index.php?title=Miniprep/GET_buffer&diff=485260&oldid=prev Michael A. Speer: /* Notes */ 2011-01-14T00:51:11Z

<p><span class="autocomment">Notes</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 00:51, 14 January 2011</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 35:</td> <td colspan="2" class="diff-lineno">Line 35:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Notes==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Notes==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[User:Michael_A._Speer|Mike]] loves this protocol so much; he wants to take it behind the middle-school and get it pregnant.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Scale-up===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Scale-up===</div></td></tr>  </table>

Michael A. Speer http://openwetware.org/index.php?title=Miniprep/GET_buffer&diff=385930&oldid=prev Michael A. Speer: /* Optional Steps */ 2010-01-30T03:40:16Z

<p><span class="autocomment">Optional Steps</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 03:40, 30 January 2010</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 31:</td> <td colspan="2" class="diff-lineno">Line 31:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Optional Steps==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Optional Steps==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*After step 3 add 10mg lysozyme and incubate for 30 mins before proceeding with step <del class="diffchange diffchange-inline">4</del>.  This step is essential for lysing gram-positive cells.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*After step 3 add 10mg lysozyme and incubate for 30 mins before proceeding with step <ins class="diffchange diffchange-inline">5</ins>.  This step is essential for lysing gram-positive cells.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*After step 9 add 400µl PCA solution to the tube, invert to mix, and centrifuge for 3 minutes at maximum speed.  Collect the upper phase by pipetting into a new tube and proceed with step 10.  This step helps remove any residual proteins.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*After step 9 add 400µl PCA solution to the tube, invert to mix, and centrifuge for 3 minutes at maximum speed.  Collect the upper phase by pipetting into a new tube and proceed with step 10.  This step helps remove any residual proteins.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>  </table>

Michael A. Speer http://openwetware.org/index.php?title=Miniprep/GET_buffer&diff=385928&oldid=prev Michael A. Speer: /* Procedure */ 2010-01-30T03:38:29Z

<p><span class="autocomment">Procedure</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 03:38, 30 January 2010</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 15:</td> <td colspan="2" class="diff-lineno">Line 15:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Centrifuge for 1 minute at maximum speed and discard the supernatant.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Centrifuge for 1 minute at maximum speed and discard the supernatant.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Add 100ul of refrigerated GET buffer to the pellet and vortex to resuspend.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Add 100ul of refrigerated GET buffer to the pellet and vortex to resuspend.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># <del class="diffchange diffchange-inline">Add 2mg of </del>SDS <del class="diffchange diffchange-inline">to 200ul </del>of <del class="diffchange diffchange-inline">room temperature </del>0.2M NaOH <del class="diffchange diffchange-inline">and vortex to mix</del>. <del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># <ins class="diffchange diffchange-inline">Freshly prepare the alkaline </ins>SDS <ins class="diffchange diffchange-inline">solution by adding 10mg SDS per ml </ins>of 0.2M NaOH <ins class="diffchange diffchange-inline">Solution</ins>.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># <del class="diffchange diffchange-inline">Apply the </del>alkaline SDS solution to the cell suspension and invert to mix. DO NOT VORTEX!  The solution should become clear.   </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># <ins class="diffchange diffchange-inline">Add 200μl of </ins>alkaline SDS solution to the cell suspension and invert to mix. DO NOT VORTEX!  The solution should become clear.   </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Add 150ul of refrigerated potassium acetate solution and invert gently to mix. DO NOT VORTEX!  A precipitate should form.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Add 150ul of refrigerated potassium acetate solution and invert gently to mix. DO NOT VORTEX!  A precipitate should form.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Store the tube on ice for 3-5 minutes.   </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Store the tube on ice for 3-5 minutes.   </div></td></tr>  </table>

Michael A. Speer http://openwetware.org/index.php?title=Miniprep/GET_buffer&diff=368424&oldid=prev Vaishnavi Ananth at 09:42, 19 November 2009 2009-11-19T09:42:41Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 09:42, 19 November 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 46:</td> <td colspan="2" class="diff-lineno">Line 46:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This protocol is based on a protocol described by Sambrook and Russell in their manual "Molecular Cloning" (2001) p:1.32-1.34  </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This protocol is based on a protocol described by Sambrook and Russell in their manual "Molecular Cloning" (2001) p:1.32-1.34  </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==<del class="diffchange diffchange-inline">BioStream </del>version==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==<ins class="diffchange diffchange-inline">BioCoder </ins>version==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Following is the Miniprep/GET Buffer protocol in <del class="diffchange diffchange-inline">BioStream</del>, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol that was coded up in <del class="diffchange diffchange-inline">BioStream </del>(see Source code). More information about <del class="diffchange diffchange-inline">BioStream </del>can be found on my home page. Feel free to mail me your comments/ suggestions.[[User:Vaishnavi Ananth|Vaishnavi]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Following is the Miniprep/GET Buffer protocol in <ins class="diffchange diffchange-inline">BioCoder</ins>, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol that was coded up in <ins class="diffchange diffchange-inline">BioCoder </ins>(see Source code). More information about <ins class="diffchange diffchange-inline">BioCoder </ins>can be found on my home page. Feel free to mail me your comments/ suggestions.[[User:Vaishnavi Ananth|Vaishnavi]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Text Output====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Text Output====</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Miniprep - GET Buffer protocol]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Miniprep - GET Buffer protocol]]</div></td></tr>  </table>

Vaishnavi Ananth http://openwetware.org/index.php?title=Miniprep/GET_buffer&diff=356860&oldid=prev Michael A. Speer at 14:46, 8 October 2009 2009-10-08T14:46:21Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 14:46, 8 October 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 23:</td> <td colspan="2" class="diff-lineno">Line 23:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Add 900ul of 100% (95% is ok too) EtOH to precipitate the plasmid DNA.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Add 900ul of 100% (95% is ok too) EtOH to precipitate the plasmid DNA.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Place the tubes in the -80 freezer for 30 minutes.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Place the tubes in the -80 freezer for 30 minutes.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Centrifuge the precipitated plasmid DNA <del class="diffchange diffchange-inline">10 </del>minutes at maximum speed and discard supernatant.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Centrifuge the precipitated plasmid DNA <ins class="diffchange diffchange-inline">15 </ins>minutes at maximum speed <ins class="diffchange diffchange-inline">(cold if possible)</ins>and discard supernatant.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Carefully add 1ml 70% EtOH to the pellet and let sit for 3 minutes.   </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Carefully add 1ml 70% EtOH to the pellet and let sit for 3 minutes.   </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Centrifuge at maximum speed for 3 minutes.  Make sure the pellet is toward the outside.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Centrifuge at maximum speed for 3 minutes.  Make sure the pellet is toward the outside.</div></td></tr> <tr><td colspan="2" class="diff-lineno">Line 36:</td> <td colspan="2" class="diff-lineno">Line 36:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Notes==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Notes==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Michael_A._Speer|Mike]] loves this protocol so much; he wants to take it behind the middle-school and get it pregnant.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Michael_A._Speer|Mike]] loves this protocol so much; he wants to take it behind the middle-school and get it pregnant.</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">===Scale-up===</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Even higher concentrations of plasmid have been obtained by scaling up this protocol 5x in 15ml centrifuge tubes.  However, to do this a larger (i.e. slower) centrifuge was necessary to accomodate the larger tubes.  The adjusted centrifugation steps were as follows:</br></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">::Step 9: Centrifuge at 5500g (Max speed for this larger centrifuge) for 15 minutes.</br></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">::Step 13: Centrifuge at 5500g for 20 minutes.</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td></tr>  </table>

Michael A. Speer http://openwetware.org/index.php?title=Miniprep/GET_buffer&diff=352533&oldid=prev Vaishnavi Ananth: /* BioStream version */ 2009-09-22T12:24:41Z

<p><span class="autocomment">BioStream version</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 12:24, 22 September 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 42:</td> <td colspan="2" class="diff-lineno">Line 42:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==BioStream version==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==BioStream version==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Following is the Miniprep/GET Buffer protocol in BioStream, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol that was coded up in BioStream. More information about BioStream can be found on my home page. Feel free to mail me your comments/ suggestions.[[User:Vaishnavi Ananth|Vaishnavi]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Following is the Miniprep/GET Buffer protocol in BioStream, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol that was coded up in BioStream <ins class="diffchange diffchange-inline">(see Source code)</ins>. More information about BioStream can be found on my home page. Feel free to mail me your comments/ suggestions.[[User:Vaishnavi Ananth|Vaishnavi]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Text Output====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Text Output====</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Miniprep - GET Buffer protocol]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Miniprep - GET Buffer protocol]]</div></td></tr>  </table>

Vaishnavi Ananth http://openwetware.org/index.php?title=Miniprep/GET_buffer&diff=352531&oldid=prev Vaishnavi Ananth at 12:17, 22 September 2009 2009-09-22T12:17:52Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 12:17, 22 September 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 44:</td> <td colspan="2" class="diff-lineno">Line 44:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Following is the Miniprep/GET Buffer protocol in BioStream, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol that was coded up in BioStream. More information about BioStream can be found on my home page. Feel free to mail me your comments/ suggestions.[[User:Vaishnavi Ananth|Vaishnavi]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Following is the Miniprep/GET Buffer protocol in BioStream, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol that was coded up in BioStream. More information about BioStream can be found on my home page. Feel free to mail me your comments/ suggestions.[[User:Vaishnavi Ananth|Vaishnavi]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Text Output====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Text Output====</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[<del class="diffchange diffchange-inline">Image:Protocol in BioStream.pdf</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[<ins class="diffchange diffchange-inline">Miniprep - GET Buffer protocol</ins>]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Source Code====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Source Code====</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Miniprep - GET Buffer protocol - source code]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Miniprep - GET Buffer protocol - source code]]</div></td></tr>  </table>

Vaishnavi Ananth http://openwetware.org/index.php?title=Miniprep/GET_buffer&diff=352528&oldid=prev Vaishnavi Ananth at 11:58, 22 September 2009 2009-09-22T11:58:50Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 11:58, 22 September 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 44:</td> <td colspan="2" class="diff-lineno">Line 44:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Following is the Miniprep/GET Buffer protocol in BioStream, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol that was coded up in BioStream. More information about BioStream can be found on my home page. Feel free to mail me your comments/ suggestions.[[User:Vaishnavi Ananth|Vaishnavi]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Following is the Miniprep/GET Buffer protocol in BioStream, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol that was coded up in BioStream. More information about BioStream can be found on my home page. Feel free to mail me your comments/ suggestions.[[User:Vaishnavi Ananth|Vaishnavi]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Text Output====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Text Output====</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:<del class="diffchange diffchange-inline">Miniprep - GET Buffer protocol</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:<ins class="diffchange diffchange-inline">Protocol in BioStream.pdf</ins>]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Source Code====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Source Code====</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Miniprep - GET Buffer protocol - source code]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Miniprep - GET Buffer protocol - source code]]</div></td></tr>  </table>

Vaishnavi Ananth http://openwetware.org/index.php?title=Miniprep/GET_buffer&diff=352527&oldid=prev Vaishnavi Ananth at 11:57, 22 September 2009 2009-09-22T11:57:43Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 11:57, 22 September 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 44:</td> <td colspan="2" class="diff-lineno">Line 44:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Following is the Miniprep/GET Buffer protocol in BioStream, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol that was coded up in BioStream. More information about BioStream can be found on my home page. Feel free to mail me your comments/ suggestions.[[User:Vaishnavi Ananth|Vaishnavi]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Following is the Miniprep/GET Buffer protocol in BioStream, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol that was coded up in BioStream. More information about BioStream can be found on my home page. Feel free to mail me your comments/ suggestions.[[User:Vaishnavi Ananth|Vaishnavi]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Text Output====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Text Output====</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Miniprep - GET Buffer protocol]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[<ins class="diffchange diffchange-inline">Image:</ins>Miniprep - GET Buffer protocol]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Source Code====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Source Code====</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Miniprep - GET Buffer protocol - source code]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Miniprep - GET Buffer protocol - source code]]</div></td></tr>  </table>

Vaishnavi Ananth http://openwetware.org/index.php?title=Miniprep/GET_buffer&diff=352522&oldid=prev Vaishnavi Ananth at 11:33, 22 September 2009 2009-09-22T11:33:03Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 11:33, 22 September 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 43:</td> <td colspan="2" class="diff-lineno">Line 43:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==BioStream version==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==BioStream version==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Following is the Miniprep/GET Buffer protocol in BioStream, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol that was coded up in BioStream. More information about BioStream can be found on my home page. Feel free to mail me your comments/ suggestions.[[User:Vaishnavi Ananth|Vaishnavi]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Following is the Miniprep/GET Buffer protocol in BioStream, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol that was coded up in BioStream. More information about BioStream can be found on my home page. Feel free to mail me your comments/ suggestions.[[User:Vaishnavi Ananth|Vaishnavi]]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>===Text Output===</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">=</ins>===Text Output<ins class="diffchange diffchange-inline">=</ins>===</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Miniprep - GET Buffer protocol]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Miniprep - GET Buffer protocol]]</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">====Source Code====</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Miniprep - GET Buffer protocol - source code]]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Protocol]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Protocol]]</div></td></tr>  </table>

Vaishnavi Ananth