Mimulus:Seed Sterilization

Seed Sterilization

• Supplies
• 70% Ethanol
• 100% Bleach
• Sterile Water
• Seeds

1. Put seeds in an Eppendorf tube
2. Add 200uL of 70% EtOH to tube
3. Immediately add 600 uL of Water

Do only 2 tubes at a time, because the alcohol treatment is harsh and might Affect germination if is left on seeds too long

1. Immediately suck off 600 uL of supernatant
2. Repeat steps 3 and 4 four times
3. Set aside tubes and repeat steps 1 to 5 for all the remaining tubes
4. Add 200 uL of 100% bleach to each tube
5. Let tubes sit for 5 minutes
6. Add 600 uL of water
7. Suck off 600 uL of the supernatant
8. Repeat steps 9 and 10 four or five times