Mike Barnkob:Protocols/Mediums: Difference between revisions
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* 5 ml of Pen/strep (100 U/mL penicillin and 100 ug/mL streptomycin) | * 5 ml of Pen/strep (100 U/mL penicillin and 100 ug/mL streptomycin) | ||
* 2 mM L-glutamine | * 2 mM L-glutamine | ||
===FACS buffer=== | |||
* 500 ml of DPBS | |||
* 2mM EDTA | |||
* 50 ml of FCS | |||
Latest revision as of 13:35, 30 April 2015
Media
Recipes for media.
R0
- 500 ml of RPMI
- 5 ml of Pen/strep (100 U/mL penicillin and 100 ug/mL streptomycin)
- 2 mM L-glutamine
R10
- 500 ml of RPMI
- 50 ml of FCS
- 5 ml of Pen/strep (100 U/mL penicillin and 100 ug/mL streptomycin)
- 2 mM L-glutamine
R10 for T cells
- 500 ml of RPMI
- 50 ml of FCS
- 5 ml of Pen/strep (100 U/mL penicillin and 100 ug/mL streptomycin)
- 2 mM L-glutamine
- 50 μM B2-M (500μL of 50mM solution)
D10
- 500 ml of DMEM
- 50 ml of FCS
- 5 ml of Pen/strep (100 U/mL penicillin and 100 ug/mL streptomycin)
- 2 mM L-glutamine
FACS buffer
- 500 ml of DPBS
- 2mM EDTA
- 50 ml of FCS
