Methods to determine the size of an object in microns

=Mathematically

Pixels on our CCD are 6.45um.

The size (in microns) of one pixel in an image will depend on how much the image was magnified before reaching the CCD:

[math]\displaystyle{ \mbox{Pixel size} = \frac{6.45um}{\mbox{total magnification}} }[/math]