# Methods to determine the size of an object in microns

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Assuming that the magnifier is at position 1x (not 1.5x), this formula gives us: | Assuming that the magnifier is at position 1x (not 1.5x), this formula gives us: | ||

- | 60x : 0.1075 um/pixel or 9.30 pixels/um | + | *60x : 0.1075 um/pixel or 9.30 pixels/um |

- | 40x : 0.1613 um/pixel or 6.21 pixels/um | + | *40x : 0.1613 um/pixel or 6.21 pixels/um |

- | 20x : 0.3225 um/pixel or 3.10 pixels/um | + | *20x : 0.3225 um/pixel or 3.10 pixels/um |

- | 10x : 0.645 um/pixel or 1.55 pixel/um | + | *10x : 0.645 um/pixel or 1.55 pixel/um |

## Revision as of 12:42, 11 August 2005

### Mathematically

Pixels on our CCD are 6.45um. The size (in microns) of one pixel in an image will depend on how much the image was magnified before reaching the CCD:

Assuming that the magnifier is at position 1x (not 1.5x), this formula gives us:

- 60x : 0.1075 um/pixel or 9.30 pixels/um
- 40x : 0.1613 um/pixel or 6.21 pixels/um
- 20x : 0.3225 um/pixel or 3.10 pixels/um
- 10x : 0.645 um/pixel or 1.55 pixel/um