# Methods to determine the size of an object in microns

(Difference between revisions)
 Revision as of 12:30, 11 August 2005 (view source)← Previous diff Revision as of 12:42, 11 August 2005 (view source) (→=Mathematically)Next diff → Line 1: Line 1: - ===Mathematically== + ===Mathematically=== - Pixels on our CCD are 6.45um. + Pixels on our CCD are 6.45um. The size (in microns) of one pixel in an image will depend on how much the image was magnified before reaching the CCD: - + - The size (in microns) of one pixel in an image will depend on how much the image was magnified before reaching the CCD: + $\mbox{Pixel size} = \frac{6.45um}{\mbox{total magnification}}$ $\mbox{Pixel size} = \frac{6.45um}{\mbox{total magnification}}$ + + Assuming that the magnifier is at position 1x (not 1.5x), this formula gives us: + + 60x  :  0.1075 um/pixel  or  9.30 pixels/um + 40x  :  0.1613 um/pixel  or  6.21 pixels/um + 20x  :  0.3225 um/pixel  or  3.10 pixels/um + 10x  :  0.645  um/pixel  or  1.55 pixel/um

## Revision as of 12:42, 11 August 2005

### Mathematically

Pixels on our CCD are 6.45um. The size (in microns) of one pixel in an image will depend on how much the image was magnified before reaching the CCD:

$\mbox{Pixel size} = \frac{6.45um}{\mbox{total magnification}}$

Assuming that the magnifier is at position 1x (not 1.5x), this formula gives us:

60x  : 0.1075 um/pixel or 9.30 pixels/um 40x  : 0.1613 um/pixel or 6.21 pixels/um 20x  : 0.3225 um/pixel or 3.10 pixels/um 10x  : 0.645 um/pixel or 1.55 pixel/um