Methods to determine the size of an object in microns

(Difference between revisions)
 Revision as of 13:30, 11 August 2005 (view source)← Previous diff Revision as of 13:42, 11 August 2005 (view source) (→=Mathematically)Next diff → Line 1: Line 1: - ===Mathematically== + ===Mathematically=== - Pixels on our CCD are 6.45um. + Pixels on our CCD are 6.45um. The size (in microns) of one pixel in an image will depend on how much the image was magnified before reaching the CCD: - + - The size (in microns) of one pixel in an image will depend on how much the image was magnified before reaching the CCD: + $\mbox{Pixel size} = \frac{6.45um}{\mbox{total magnification}}$ $\mbox{Pixel size} = \frac{6.45um}{\mbox{total magnification}}$ + + Assuming that the magnifier is at position 1x (not 1.5x), this formula gives us: + + 60x  :  0.1075 um/pixel  or  9.30 pixels/um + 40x  :  0.1613 um/pixel  or  6.21 pixels/um + 20x  :  0.3225 um/pixel  or  3.10 pixels/um + 10x  :  0.645  um/pixel  or  1.55 pixel/um

Revision as of 13:42, 11 August 2005

Mathematically

Pixels on our CCD are 6.45um. The size (in microns) of one pixel in an image will depend on how much the image was magnified before reaching the CCD:

$\mbox{Pixel size} = \frac{6.45um}{\mbox{total magnification}}$

Assuming that the magnifier is at position 1x (not 1.5x), this formula gives us:

60x  : 0.1075 um/pixel or 9.30 pixels/um 40x  : 0.1613 um/pixel or 6.21 pixels/um 20x  : 0.3225 um/pixel or 3.10 pixels/um 10x  : 0.645 um/pixel or 1.55 pixel/um