McClean:dNTPs: Difference between revisions
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(New page: We use Roche Diagnostics dNTP set. Each individual dNTP comes as 10 μmol in 100 mM solution for a total of 4 tubes. Each lids labeled with the nucleic acid it contains. The kits are ...) |
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Revision as of 16:36, 8 August 2011
We use Roche Diagnostics dNTP set. Each individual dNTP comes as 10 μmol in 100 mM solution for a total of 4 tubes. Each lids labeled with the nucleic acid it contains. The kits are from the MolBio stock room (order number FZ129). We use our dNTPS at 10 mM so we need to dilute them 1:10.
dNTP stocks are kept at 10 mM:
- Add 3.6 mL of dH20 to a falcon tube.
- Add the full 100 μL of each nucleotide to the tube (after all 4 have been added should have a total volume of 4 mL)
- Aliquot 50 uL of dNTPs into 1.5 mL eppendorf tubes and label as "dNTPs 10 mM" and store in the -80°C fridge.
Most recent stock made by Anjali Bisaria 8 Aug 2011. Lot Number: 12659700
