McClean: Washing Glassware

Read the following (especially the protocol from Sigma Aldrich)

• http://www.aceglass.com/dpro/kb_article.php?ref=8386-IPSF-3037
• http://csmedia2.corning.com/LifeSciences/Media/pdf/cls_an_112_cleaningglassware.pdf

• https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Aldrich/Bulletin/al_techbull_al228.pdf

• http://labprotocolsonline.blogspot.com/2011/06/caring-and-cleaning-lab-glassware.html

Note that the autoclave that needs to be used for sterilizing all glassware that will be used for microbiology is in the BME Shared Lab (2046). It has a setting for glassware, which is ?? (someone walk down, read it, and put that info in once we figure it out).

Cleaning glassware:

Pre-Autoclaving:

Before autoclaving, soak the glassware in a plastic tub of hot water and Alconox for about 1 hour. If the glassware is noticeably dirty, use the scrub to get rid of the residue. Make sure the scrub is not worn out so the spine hits the glass. This will scratch the glass. If it is worn out, get a new scrub. After it is done soaking, rinse them in the sink with tap water to get rid of the Alconox. Then, rinse with de-ionized water (MilliQ water). Use the drying rack to dry them. If the glassware does not fit, dry them in another plastic tub laid with paper towels or a clean cloth.

The glassware should be dried over night. Once they are dried, they are ready to be autoclaved.

Autoclaving:

The top of the glassware should be wrapped in aluminum foil and autoclave tape. They should be put into the autoclave cart and wheeled down to 2046.