McClean: Potassium Phosphate: Difference between revisions
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==Procedure== | ==Procedure== | ||
==Stock Solutions== | ==Stock Solutions== |
Revision as of 07:51, 2 July 2015
Overview
Potassium phosphate for several procedures including fixing yeast cells for fluorescence measurements.
Materials
- Potassium phosphate monobasic KH2PO4
- Potassium phosphate dibasic K2HPO4
Procedure
Stock Solutions
Please write in here when you make a new stock solution and how you made it, how many tubes, etc. Also add a note to the "Yeast Media Antibiotic Concentrations" sheet on the front of the 4°C fridge.
*Megan N McClean Made stock on 11/30/2011. Geneticin Lot #919813, activity 701ug/mg. 1000/701*200= 285.3067; 1000/ 285.3067=3.505ml. Dissolve contents of the entire 1g vial in 3.505ml total volume of 1x PBS. Sterile-filted, aliquoted in 500ul in eppendorfs. Left one tube at 4°C and put the others at -80°C
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
Contact
- Megan N McClean 14:01, 23 September 2011 (EDT)
or instead, discuss this protocol.