Megan N McClean at 19:07, 13 December 2011

Megan N McClean — Tue, 13 Dec 2011 19:07:04 GMT

←Older revision		Revision as of 19:07, 13 December 2011
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-	<!-- COPY ~~EVERYHING~~ BELOW HERE TO START YOUR OWN PROTOCOL! -->	+	<!-- COPY EVERYTHING BELOW HERE TO START YOUR OWN PROTOCOL! -->

	==Overview==		==Overview==
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	==Protocol==		==Protocol==
	* Open the Windows ''Start'' menu		* Open the Windows ''Start'' menu
-	*Choose ''All Programs''	+	* Choose ''All Programs''
	* Choose ''NIS-Elements 3.2 64-bit''		* Choose ''NIS-Elements 3.2 64-bit''
-	*Choose ''Modify Installation''	+	* Choose ''Modify Installation''
-	Click Repair, Next	+	* Click ''Repair'' and choose ''Next''
		+	* Click ''Next'' or ''Finish'' as necessary to run the utility
		+	* When prompted to restart the computer, do so.

-	~~If prompted to restart, restart the computer~~
	==Notes==		==Notes==
	<!-- Please paste this section "as is" into your protocol, and add notes to it if you have some!-->		<!-- Please paste this section "as is" into your protocol, and add notes to it if you have some!-->
	Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input! Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.		Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input! Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.
-	#'''*[[User:Megan N McClean\|Megan N McClean]]''' ~~When I can't age my dissection plates on my bench for a few days, I will stick them in~~ the ~~30°C or 37°C warm room~~ the ~~morning of~~ the ~~day I dissect~~ to ~~dry them out a little bit~~. ~~It is absolutely infuriating, if not impossible,~~ to ~~try dissection on plates~~ that ~~are wet.~~	+	#'''*[[User:Megan N McClean\|Megan N McClean]]''' The "stage crashing all the way to the bottom left" error is particularly disturbing as when this happens the stage crashes into whatever objective happens to be in position. If you have any reason to believe that the scope is going to misbehave please remove the objectives before continuing. A particularly good time to worry about this and remove the objectives before messing with stage positions is if you've been messing with any drivers or installing drivers that might conflict with the NIS elements software.
-	~~#'''*[[User:Megan N McClean\|Megan N McClean]]''' Different tetrad dissection protocols call for using different enzymes to digest~~ the ~~ascus wall. Our protocol uses a β-glucuronidase from Sigma (G7770) which~~ is ~~a mixture of enzymes derived from Helix pomatia (~~the ~~Roman snail)~~. Zymolyase, another commonly used enzyme, consists mostly of β-1,3-glucan laminaripentaohydrolase. It hydrolyzes glucose polymers at the β-1,3-glucan linkages releasing laminaripentaose as the principal product. ~~β-glucuronidase catalyzes hydrolysis of β-D-glucuronic acid residues from~~ the ~~non-reducing end of mucopolysaccharides (also referred to as glycosaminoglycans).~~	+
-		+
-	~~==References==~~	+
-	~~Adapted from Maitreya Dunham~~'~~s protocol (http://dunham.gs.washington.edu/sporulationdissection.htm) and~~ the ~~Botstein lab protocol (http://www.princeton.edu/genomics/botstein/protocols/Sporulation_and_Tetrad_Dissection~~.~~pdf)~~	+

	==Contact==		==Contact==

Megan N McClean: New page: ==Overview== On occasion "funny" things happen with the NIS Elements software/drivers. For example, every time you mark a ...

Megan N McClean — Tue, 13 Dec 2011 18:59:38 GMT

New page:  ==Overview== On occasion "funny" things happen with the NIS Elements software/drivers. For example, every time you mark a ...

New page

==Overview==
On occasion "funny" things happen with the NIS Elements software/drivers. For example, every time you mark a stage position the stage goes all the way to the left hand bottom corner regardless of what position you marked. When this happens it is time to run the repair utility.

==Protocol==
* Open the Windows ''Start'' menu
*Choose ''All Programs''
* Choose ''NIS-Elements 3.2 64-bit''
*Choose ''Modify Installation''
Click Repair, Next

If prompted to restart, restart the computer
==Notes==

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input! Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.
#'''*[[User:Megan N McClean|Megan N McClean]]''' When I can't age my dissection plates on my bench for a few days, I will stick them in the 30°C or 37°C warm room the morning of the day I dissect to dry them out a little bit. It is absolutely infuriating, if not impossible, to try dissection on plates that are wet.
#'''*[[User:Megan N McClean|Megan N McClean]]''' Different tetrad dissection protocols call for using different enzymes to digest the ascus wall. Our protocol uses a β-glucuronidase from Sigma (G7770) which is a mixture of enzymes derived from Helix pomatia (the Roman snail). Zymolyase, another commonly used enzyme, consists mostly of β-1,3-glucan laminaripentaohydrolase. It hydrolyzes glucose polymers at the β-1,3-glucan linkages releasing laminaripentaose as the principal product. β-glucuronidase catalyzes hydrolysis of β-D-glucuronic acid residues from the non-reducing end of mucopolysaccharides (also referred to as glycosaminoglycans).

==References==
Adapted from Maitreya Dunham's protocol (http://dunham.gs.washington.edu/sporulationdissection.htm) and the Botstein lab protocol (http://www.princeton.edu/genomics/botstein/protocols/Sporulation_and_Tetrad_Dissection.pdf)

==Contact==

*'''[[User:Megan N McClean|Megan N McClean]] 14:01, 20 July 2011 (EDT)'''

or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].


[[Category:Protocol]]

McClean: NIS Elements Repair - Revision history

Megan N McClean at 19:07, 13 December 2011

Megan N McClean: New page: ==Overview== On occasion "funny" things happen with the NIS Elements software/drivers. For example, every time you mark a ...