McClean: Lab Organization Notes: Difference between revisions
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[[Image:McClean_whitefreezerbox.jpg|thumb|200px|center|Use the white cardboard boxes for personal storage in your 4°C, -20°C, and -80°C storage locations. These are also the preferred boxes for storing common stocks (competent cells, PCR buffers, etc) in all storage locations.]] | [[Image:McClean_whitefreezerbox.jpg|thumb|200px|center|Use the white cardboard boxes for personal storage in your 4°C, -20°C, and -80°C storage locations. These are also the preferred boxes for storing common stocks (competent cells, PCR buffers, etc) in all storage locations.]] | ||
[[Image:McClean_GrayFreezerBox.jpg |thumb|200px|center|Use the gray freezer boxes (Nunc #374187) for primers (100μM stock) in the -80°C and for plasmids and oligos in the -20°C. DO NOT use these for any other storage purposes.]] | [[Image:McClean_GrayFreezerBox.jpg |thumb|200px|center|Use the gray freezer boxes (Nunc #374187) for primers (100μM stock) in the -80°C and for plasmids and oligos in the -20°C. DO NOT use these for any other storage purposes.]] | ||
[[Image:McClean_ClearFreezerBox.jpg|thumb|300px|center|Use these for storing yMMs or bMMs in the -80°C. Do not use for any other purpose]] | [[Image:McClean_ClearFreezerBox.jpg|thumb|300px|center|Use these for storing yMMs or bMMs in the -80°C. Do not use for any other purpose. VWR #40000-320]] | ||
We also have a large collection of various plastic freezer/storage boxes that we've accumulated over the years. These can be used for storage of common reagents in the 4°C, -20°C, and -80°C spaces. Be sure to label and date everything that you put into storage. You should keep careful records of what you store in your own storage boxes. | We also have a large collection of various plastic freezer/storage boxes that we've accumulated over the years. These can be used for storage of common reagents in the 4°C, -20°C, and -80°C spaces. Be sure to label and date everything that you put into storage. You should keep careful records of what you store in your own storage boxes. | ||
==The Where=== | ==The Where=== | ||
Revision as of 12:01, 2 September 2015
The Which
Which box should I use for storing my items?
We also have a large collection of various plastic freezer/storage boxes that we've accumulated over the years. These can be used for storage of common reagents in the 4°C, -20°C, and -80°C spaces. Be sure to label and date everything that you put into storage. You should keep careful records of what you store in your own storage boxes.
The Where=
Where to put things in the -80°C Freezers=
- Store personal reagents in white freezer boxes (see picture) and store in your rack (see freezer chart here: )
- yMMs, bMMs
- You will always freeze a main and a backup copy of y, -MMs and bMMs. The main copy goes in the main -80°C freezer () the copy goes in the backup -80°C
