McClean: Frogging a Serial Dilution

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Contents

Overview

Frogging a serial dilution onto solid media is an effective way to distinguish and compare viability under certain growth conditions, etc. The serial dilution helps to eliminate the possibility that a relatively high cell concentration could be misinterpreted as healthy growth, for example.

Materials

  • 96-well plate (flat bottom wells)
  • "Frogger"
  • Sterile petri dish about half-full of 95% ethanol
  • Sterile petri dish

Stock Solutions

Stock Solution 1

  • This is a very simple solution, so we only need a one line description of how to make it.

Stock Solution 2

This is a more involved solution, so we will describe how to make it in several steps:

  1. Step 1
  2. Step 2
  3. Step 3

Protocol

  1. Step 1
  2. Step 2
  3. Step 3


Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

Gietz, R.D. and R.A. Woods. (2002) TRANSFORMATION OF YEAST BY THE Liac/SS CARRIER DNA/PEG METHOD. Methods in Enzymology 350: 87-96.

Contact

or instead, discuss this protocol.

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