McClean: Flow Cells

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Contents

Overview

This protocol covers the soft lithography and plasma bonding steps used to make flow cells out of PDMS in our lab.

Materials

  • Slygard

• 4” petri dishes (for storing chips) • small ~6” pieces of the intramedic tubing (ID 0.86mm OD 1.27mm) • razor blades • Nitrile gloves • Biopsy Punches (1.2mm, 1.0mm, 0.75mm diameters) • Stainless steel blunt needle, 16 ½” gauge • Small green needle (21 ½ gauge Becton-Dickinson) • Pieces of scrap PDMS (in small petri dishes on your bench) • Cured PDMS, cut from molds (in 4” petri dishes, covered with tape) • Scotch tape • 1ml syringes with Luer-Lok tips • 4” petri dishes • 4” silicon wafers • SU8-20205 photoresist • SU8 developer (propylene glycol monomethyl ether acetate PGMEA) • Transparency Masks • Wafer Tweezers • Scotch tape • Scissors • Transparency Masks • Aluminum foil • Acetone • Cleanroom wipes (do not shed particles like paper towels or Kim wipes) • HMDS (hexamethyldisilazane) • 5” square glass plates with rounded edges • Lab coat • Hairnets • nitrile glocse • Cleanroom booties • Safety glasses • Sheet protectors for holding protocols • Cleanroom paper for taking notes • Timers • Slygard 184 Silicon Elastromer • Rough PDMS chips cut from molds • 1.5mm Coverslips • Oven set to 65°C (Microarray hybridization oven) • Scotch tape • Nitrile gloves • TMCS (chlorotrimethyl silane) • plastic forks for mixing PDMS • plastic beakers for mixing PDMS • Vacumn jar for degassing PDMS • Microfluidic chips constructed previously • Tubing • Microscopes • Fluoresceine solution in water • rhodamine solution in water • 15ml conical tubes • Syringes with tubing for injecting the chips. • 70% ethanol in a 50ml conical • Deionized water in a 50ml conical


Protocol

Mixing the PDMS

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
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References

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or instead, discuss this protocol.

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