McClean:Yeast Transformation
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Overview
Our lab's version of the Geitz lithium-acetate transformation method.
Materials
- 1M LiAC
- 100mM LiAC
- 50% w/v PEG (mw )
- Sterile H20
- Appropriate selective plates (SC-URA, YPD+G418, etc)
Day 1
- Inoculate the strain to transform from a single colony into 5mls of YPD in a test tube. Put on the roller drum at 30°C overnight.
Day 2
Notes
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References
Gietz, R.D. and R.A. Woods. (2002) TRANSFORMATION OF YEAST BY THE Liac/SS CARRIER DNA/PEG METHOD. Methods in Enzymology 350: 87-96.
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.