McClean:YPD4: Difference between revisions
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(New page: ==Overview== YPD liquid media ==Reagents== * Bacto yeast extract * Bacto peptone * MilliQ H<sub>2</sub>O ==Protocol== *Begin with approximately 700mls of MiliQ water *Weigh out and add i...) |
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* Bacto peptone | * Bacto peptone | ||
* MilliQ H<sub>2</sub>O | * MilliQ H<sub>2</sub>O | ||
* Dextrose | |||
==Protocol== | ==Protocol== | ||
Revision as of 10:18, 2 July 2015
Overview
YPD liquid media
Reagents
- Bacto yeast extract
- Bacto peptone
- MilliQ H2O
- Dextrose
Protocol
- Begin with approximately 700mls of MiliQ water
- Weigh out and add ingredients with constant stirring:
- Bacto Yeast Extract 10g
- Bacto Peptone 20g
- Bring up to 900mls with MiliQ water
- Weigh out 20g of glucose/dextrose and add to 100mls of MiliQ water (total volume)
- Autoclave separately
- Allow to cool in 55°C water batch
- Combine YP with glucose solution just because you are ready to use the media for the first time.
- Container 2:
- Begin with approx. 80ml MilliQ H2O
- Add with constant stirring 6.7g of Yeast Nitrogen base (without amino acids)
- Bring up to 100ml with MilliQ H2O
- Autoclave
- Allow to cool (50°F)
Combine contents of 2 containers and mix well
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
References
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
