McClean:YPD2: Difference between revisions
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(New page: ==Overview== Yeast nitrogen base without amino acids with agar for plates. This protocol leaves 100ml (per L) of room for additives (glucose, amino acids, etc). Media is labelled as YNB1...) |
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==Overview== | ==Overview== | ||
YPD agar for plates. | |||
==Reagents== | ==Reagents== | ||
* | * Bacto yeast extract | ||
* Bacto | * Bacto Peptone | ||
* MilliQ H<sub>2</sub>O | * MilliQ H<sub>2</sub>O | ||
* Dextrose | |||
==Protocol== | ==Protocol== | ||
*Begin with approximately 700mls of MiliQ water | |||
*Weigh out and add ingredients with constant stirring: | |||
* | **Bacto yeast extract 10g | ||
** | **Bacto peptone 20g | ||
** | *Weigh out and add with stirring: | ||
* | *Bacto agar 20g | ||
*Bring up to 900mls with MiliQ water | |||
* | *Separate YP into two 1L bottles of 450mls each | ||
* | *Weight out 20g of dextrose and add to 60mls of MiliQ water and bring volume up to 100ml total | ||
* | *Autoclave separately | ||
* | *Allow to coole in 55°C water bath | ||
*Combine each 450mls YP with 50mls of glucose solution | |||
Combine | |||
==Notes== | ==Notes== |
Revision as of 10:13, 2 July 2015
Overview
YPD agar for plates.
Reagents
- Bacto yeast extract
- Bacto Peptone
- MilliQ H2O
- Dextrose
Protocol
- Begin with approximately 700mls of MiliQ water
- Weigh out and add ingredients with constant stirring:
- Bacto yeast extract 10g
- Bacto peptone 20g
- Weigh out and add with stirring:
- Bacto agar 20g
- Bring up to 900mls with MiliQ water
- Separate YP into two 1L bottles of 450mls each
- Weight out 20g of dextrose and add to 60mls of MiliQ water and bring volume up to 100ml total
- Autoclave separately
- Allow to coole in 55°C water bath
- Combine each 450mls YP with 50mls of glucose solution
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
References
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.