McClean:YPD2: Difference between revisions
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**Bacto peptone 20g | **Bacto peptone 20g | ||
*Weigh out and add with stirring: | *Weigh out and add with stirring: | ||
*Bacto agar 20g | **Bacto agar 20g | ||
*Bring up to 900mls with MiliQ water | *Bring up to 900mls with MiliQ water | ||
*Separate YP into two 1L bottles of 450mls each | *Separate YP into two 1L bottles of 450mls each | ||
*Weight out 20g of dextrose and add to | *Weight out 20g of dextrose and add to 80mls of MiliQ water and bring volume up to 100ml total | ||
*Autoclave separately | *Autoclave separately | ||
*Allow to | *Allow to cool in 55°C water bath | ||
*Combine each 450mls YP with 50mls of glucose solution | *Combine each 450mls YP with 50mls of glucose solution | ||
Latest revision as of 10:18, 2 July 2015
Overview
YPD agar for plates.
Reagents
- Bacto yeast extract
- Bacto Peptone
- MilliQ H2O
- Dextrose
Protocol
- Begin with approximately 700mls of MiliQ water
- Weigh out and add ingredients with constant stirring:
- Bacto yeast extract 10g
- Bacto peptone 20g
- Weigh out and add with stirring:
- Bacto agar 20g
- Bring up to 900mls with MiliQ water
- Separate YP into two 1L bottles of 450mls each
- Weight out 20g of dextrose and add to 80mls of MiliQ water and bring volume up to 100ml total
- Autoclave separately
- Allow to cool in 55°C water bath
- Combine each 450mls YP with 50mls of glucose solution
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
References
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
