McClean:Western Blot

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==Overview==
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This is a template for how to write a new protocol in openwetware for our lab.  In your real protocol, a description of what the protocol is and when to use it would go here.
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==Materials==
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* Item 1
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* Item 2
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* Stock Solution 1
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* Stock Solution 2
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==Stock Solutions==
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'''Stock Solution 1'''
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* This is a very simple solution, so we only need a one line description of how to make it.   
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'''Stock Solution 2'''
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This is a more involved solution, so we will describe how to make it in several steps:
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# Step 1
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# Step 2
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# Step 3
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==Protocol==
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# Step 1
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# Step 2
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# Step 3
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==Notes==
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<!-- Please paste this section "as is" into your protocol, and add notes to it if you have some!-->
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Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
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#List troubleshooting tips here. 
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#You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
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#Anecdotal observations that might be of use to others can also be posted here. 
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Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.
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==References==
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Gietz, R.D. and R.A. Woods. (2002) TRANSFORMATION OF YEAST BY THE Liac/SS
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CARRIER DNA/PEG METHOD. Methods in Enzymology 350: 87-96.
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==Contact==
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<!--Change the information below to your info if you add a new protocol-->
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*'''[[User:Megan N McClean|Megan N McClean]] 14:01, 20 July 2011 (EDT)'''
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or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].
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<!-- You can tag this protocol with various categories.  See the [[Categories]] page for more information. -->
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[[Category:Protocol]]
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<!-- Move the relevant categories above this line to tag your protocol with the label
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[[Category:Protocol]]
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[[Category:Media]]
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[[Category:Yeast]]
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-->

Revision as of 11:41, 30 May 2013

Contents

Overview

This is a template for how to write a new protocol in openwetware for our lab. In your real protocol, a description of what the protocol is and when to use it would go here.

Materials

  • Item 1
  • Item 2
  • Stock Solution 1
  • Stock Solution 2


Stock Solutions

Stock Solution 1

  • This is a very simple solution, so we only need a one line description of how to make it.

Stock Solution 2

This is a more involved solution, so we will describe how to make it in several steps:

  1. Step 1
  2. Step 2
  3. Step 3

Protocol

  1. Step 1
  2. Step 2
  3. Step 3


Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

Gietz, R.D. and R.A. Woods. (2002) TRANSFORMATION OF YEAST BY THE Liac/SS CARRIER DNA/PEG METHOD. Methods in Enzymology 350: 87-96.

Contact

or instead, discuss this protocol.

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