McClean:Smartstat Startup: Difference between revisions
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==Overview== | ==Overview== | ||
This is a basic guide to setting up the smartstat/ministat and getting it to steady state. | This is a basic guide to setting up the smartstat/ministat and getting it to steady state. | ||
= | ==Tubing Check== | ||
Make sure the following tubes are connected: | |||
=Reactor Vessel= | |||
# Effluent tube with female quick connect. | |||
# Aeration tube with small air filter, quick connect side away from vessel. | |||
# Media in tube with peristaltic pump tubing appropriate to your pump and male quick connect at the end of the tube. | |||
# Inoculation port tube with plastic tubing clamp at the end of a very small length of tubing. | |||
=Media Supply= | |||
# Vacuum tube with small air filter. | |||
# Media tube connected to metal rod reaching down to the bottom of the vessel with tubing at bottom. Male quick connect at the end of the media tube. | |||
# Media filtration tube with short length of tubing and connection for sterile vacuum filter. | |||
# Tape rubber stopper securely with electrical tape several times around prior to use. Vessel will be under pressure. | |||
=Effluent Bottle= | |||
# Sort length of tubing on Erlenmeyer with side port. | |||
# Rubber stopper with male quick connect stuck into top. | |||
# Tape down stopper with electrical tape prior to use as vessel will be under pressure. | |||
==Autoclave== | |||
Revision as of 11:42, 6 June 2012
Overview
This is a basic guide to setting up the smartstat/ministat and getting it to steady state.
Tubing Check
Make sure the following tubes are connected:
Reactor Vessel
- Effluent tube with female quick connect.
- Aeration tube with small air filter, quick connect side away from vessel.
- Media in tube with peristaltic pump tubing appropriate to your pump and male quick connect at the end of the tube.
- Inoculation port tube with plastic tubing clamp at the end of a very small length of tubing.
Media Supply
- Vacuum tube with small air filter.
- Media tube connected to metal rod reaching down to the bottom of the vessel with tubing at bottom. Male quick connect at the end of the media tube.
- Media filtration tube with short length of tubing and connection for sterile vacuum filter.
- Tape rubber stopper securely with electrical tape several times around prior to use. Vessel will be under pressure.
Effluent Bottle
- Sort length of tubing on Erlenmeyer with side port.
- Rubber stopper with male quick connect stuck into top.
- Tape down stopper with electrical tape prior to use as vessel will be under pressure.