McClean:Protocols: Difference between revisions
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==Yeast== | ==Yeast== | ||
*[[McClean: Basic_Chemostat_Guide | Basic Chemostat Guide]] | *[[McClean:Basic_Chemostat_Guide | Basic Chemostat Guide]] | ||
*[[McClean: Bayanus Transformation | Bayanus Transformation]] | *[[McClean:Bayanus Transformation | Bayanus Transformation]] | ||
*[[McClean: Cleaning Floating Pin Replicators | Cleaning Floating Pin Replicators]] | *[[McClean:Cleaning Floating Pin Replicators | Cleaning Floating Pin Replicators]] | ||
*[[McClean: Colony PCR (Yeast) | Colony PCR (Yeast)]] | *[[McClean:Colony PCR (Yeast) | Colony PCR (Yeast)]] | ||
*[[McClean: FISH | Fluorescence in situ Hybridization (Colin)]] | *[[McClean:FISH | Fluorescence in situ Hybridization (Colin)]] | ||
*[[McClean: FISH (Ping) | Fluorescent in situ Hybridization (Ping)]] | *[[McClean:FISH (Ping) | Fluorescent in situ Hybridization (Ping)]] | ||
*[[McClean: Frogging_Tetrads | Frogging Tetrads]] | *[[McClean:Frogging_Tetrads | Frogging Tetrads]] | ||
*[[McClean: Mating_Type_Testers | Mating Type Testers]] | *[[McClean:Mating_Type_Testers | Mating Type Testers]] | ||
*[[McClean: Pinning (96 or 384 format) | Pinning (96 or 384 format)]] | *[[McClean:Pinning (96 or 384 format) | Pinning (96 or 384 format)]] | ||
*[[McClean:Random_Spore_Prep |Random Spore Prep]] | *[[McClean:Random_Spore_Prep |Random Spore Prep]] | ||
*[[McClean: Sporulation | Sporulation]] | *[[McClean:Sporulation | Sporulation]] | ||
*[[McClean: Tetrad Dissection | Tetrad Dissection]] | *[[McClean:Tetrad Dissection | Tetrad Dissection]] | ||
*[[McClean: URA Pop-out | URA Pop-out]] | *[[McClean:URA Pop-out | URA Pop-out]] | ||
*[[McClean: Yeast Mating Halo Assay | Yeast Mating Halo Assay]] | *[[McClean:Yeast Mating Halo Assay | Yeast Mating Halo Assay]] | ||
*[[McClean: Yeast Nomenclature | Yeast Nomenclature]] | *[[McClean:Yeast Nomenclature | Yeast Nomenclature]] | ||
*[[McClean: Yeast Recombinational Cloning | Yeast Recombinational Cloning]] | *[[McClean:Yeast Recombinational Cloning | Yeast Recombinational Cloning]] | ||
*[[McClean: Yeast Transformation | Yeast Transformation (S. cerevisiae)]] | *[[McClean:Yeast Transformation | Yeast Transformation (S. cerevisiae)]] | ||
*[[McClean: Chemostat_Metabolic_Cycle | YMC Induction in Chemostat]] | *[[McClean:Chemostat_Metabolic_Cycle | YMC Induction in Chemostat]] | ||
*[[McClean: Zygote Picking| Zygote Picking]] | *[[McClean:Zygote Picking| Zygote Picking]] | ||
==Bacteria== | ==Bacteria== | ||
Revision as of 06:33, 5 June 2012
Back to McClean Lab
Basics
- Joining the Lab
- Leaving the Lab
- Lab Notebooks
- Dilution of Oligos
- Ordering Media
- Protocol Template
- Lab Database
Yeast
- Basic Chemostat Guide
- Bayanus Transformation
- Cleaning Floating Pin Replicators
- Colony PCR (Yeast)
- Fluorescence in situ Hybridization (Colin)
- Fluorescent in situ Hybridization (Ping)
- Frogging Tetrads
- Mating Type Testers
- Pinning (96 or 384 format)
- Random Spore Prep
- Sporulation
- Tetrad Dissection
- URA Pop-out
- Yeast Mating Halo Assay
- Yeast Nomenclature
- Yeast Recombinational Cloning
- Yeast Transformation (S. cerevisiae)
- YMC Induction in Chemostat
- Zygote Picking
Bacteria
PCR
Flow Cytometry
- General Flow Cytometry Procedure
- Cycloheximide Concentration Assay
- Flourecent Protein Folding Time Assay
- Induction time course
- Phase Plane Mapping
Media
- KS Amino Acid Supplement
- Low Fluorescence Media
- Low Fluorescence Agar Membranes
- Magic Marker Medias
- Quickie YPD supplemented with Adenine
- Synthetic Complete (SC) Yeast Media
- Synthetic Complete (SC) Media w/Drugs
Stock Solutions
- Agarose for gels
- α-Factor 1mg/ml Stock
- Bacterial Glycerol 65%
- Carbenicillin Stock Solution
- Concanavalin A Solution without ions
- Cycloheximide Stock Solution
- DNA Ladder Stock for PCR
- Geneticin/G418 Stock Solution
- PBS/0.1%Tween
- Phosphate Buffered Saline (PBS, 10x)
- Seventy Percent (70%) Ethanol
Microfluidics
Microscopy
- Microscope Dos and Don'ts
- Nikon TI Basic Use
- 96 Well Plate Assay
- NIS Elements Repair
- New Scope Settings
- Quickie ImageJ Quantification
