McClean:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(→Misc) |
(→PCR) |
||
| Line 48: | Line 48: | ||
*[[McClean:Touchdown_PCR | Touchdown PCR]] | *[[McClean:Touchdown_PCR | Touchdown PCR]] | ||
*[[McClean: Takara PrimeStar PCR | Takara PrimeStar PCR]] | *[[McClean: Takara PrimeStar PCR | Takara PrimeStar PCR]] | ||
*[[McClean: Anneal and Extend | Anneal and Extend]] | |||
==Flow Cytometry== | ==Flow Cytometry== | ||
Revision as of 14:20, 19 September 2012
Back to McClean Lab
Basics
- Joining the Lab
- Leaving the Lab
- Lab Notebooks
- Dilution of Oligos
- Ordering Media
- Protocol Template
- Lab Database
General Lab Procedures
Yeast
- Basic Chemostat Guide
- Bayanus Transformation
- Cleaning Floating Pin Replicators
- Colony PCR (Yeast)
- Fluorescence in situ Hybridization (Colin)
- Fluorescent in situ Hybridization (Ping)
- Frogging Tetrads
- Genomic DNA Prep (Bust 'n' Grab Protocol)
- GEV Strain Construction
- Mating Type Testers
- Phluorin Calibration
- Pinning (96 or 384 format)
- Random Spore Prep
- Smartstat Startup
- Sporulation
- Tetrad Dissection
- URA Pop-out
- Yeast Mating Halo Assay
- Yeast Nomenclature
- Yeast Recombinational Cloning
- Yeast Transformation (S. cerevisiae)
- YMC Induction in Chemostat
- Zygote Picking
Bacteria
PCR
Flow Cytometry
- General Flow Cytometry Procedure
- Cycloheximide Concentration Assay
- Flourecent Protein Folding Time Assay
- Induction time course
- Phase Plane Mapping
Media
- KS Amino Acid Supplement
- Low Fluorescence Media
- Low Fluorescence Agar Membranes
- Magic Marker Medias
- Quickie YPD supplemented with Adenine
- Synthetic Complete (SC) Yeast Media
- Synthetic Complete (SC) Media w/Drugs
Stock Solutions
- Agarose for gels
- α-Factor 1mg/ml Stock
- Bacterial Glycerol 65%
- Carbenicillin Stock Solution
- Concanavalin A Solution without ions
- Cycloheximide Stock Solution
- DNA Ladder Stock for PCR
- Geneticin/G418 Stock Solution
- PBS/0.1%Tween
- Phosphate Buffered Saline (PBS, 10x)
- Seventy Percent (70%) Ethanol
Microfluidics
Microscopy
- Microscope Dos and Don'ts
- Nikon TI Basic Use
- Registering objectives on the Nikon
- 96 Well Plate Assay
- NIS Elements Repair
- New Scope Settings
- Quickie ImageJ Quantification
