McClean:Protocols: Difference between revisions
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*[[McClean: Protocol Template| Protocol Template]] | *[[McClean: Protocol Template| Protocol Template]] | ||
*[[McClean: Lab Database | Lab Database]] | *[[McClean: Lab Database | Lab Database]] | ||
==Introductory Exercises== | |||
*[[McClean: Intro_to_yeast | Introduction to Yeast]] | |||
==General Lab Procedures== | ==General Lab Procedures== | ||
| Line 15: | Line 19: | ||
*[[McClean: Dry Ice-Ethanol Bath | Dry Ice-Ethanol Bath]] | *[[McClean: Dry Ice-Ethanol Bath | Dry Ice-Ethanol Bath]] | ||
*[[McClean: Pouring Gels for Electrophoresis | Pouring Gels for Electrophoresis (Mike)]] | *[[McClean: Pouring Gels for Electrophoresis | Pouring Gels for Electrophoresis (Mike)]] | ||
*[[McClean: Nanodrop2000 | Using the Nanodrop2000]] | |||
==Yeast== | ==Yeast== | ||
| Line 31: | Line 37: | ||
*[[McClean: GEV Strain Construction | GEV Strain Construction]] | *[[McClean: GEV Strain Construction | GEV Strain Construction]] | ||
*[[McClean: Glycerol stocks (yeast) | Glycerol stocks (yeast)]] | *[[McClean: Glycerol stocks (yeast) | Glycerol stocks (yeast)]] | ||
*[[McClean: Hemacytometer protocol for yeast | Hemacytometer protocol for yeast]] | |||
*[[McClean:Making and Using Frozen Yeast Competant Cells|Making and Using Frozen Yeast Competant Cells]] | *[[McClean:Making and Using Frozen Yeast Competant Cells|Making and Using Frozen Yeast Competant Cells]] | ||
*[[McClean:Mating_Type_Testers | Mating Type Testers]] | *[[McClean:Mating_Type_Testers | Mating Type Testers]] | ||
| Line 60: | Line 67: | ||
*[[McClean:Colony PCR(E. coli) | Colony PCR (''E. coli'')]] | *[[McClean:Colony PCR(E. coli) | Colony PCR (''E. coli'')]] | ||
*[[McClean:E. coli Electroporation | E. coli Electroporation]] | *[[McClean:E. coli Electroporation | E. coli Electroporation]] | ||
*[[McClean: PlasmidPrep_QiagenKit | Plasmid Prep from E. coli using a Qiagen Kit]] | |||
==PCR== | ==PCR== | ||
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*[[McClean: TAE_10X | TAE Buffer (10X)]] | *[[McClean: TAE_10X | TAE Buffer (10X)]] | ||
*[[McClean: TE_10x | TE Buffer (10X)]] | *[[McClean: TE_10x | TE Buffer (10X)]] | ||
*[[McClean: Tris_Buffer | Tris Buffer]] | |||
==Microfluidics== | ==Microfluidics== | ||
Revision as of 11:57, 27 April 2015
Back to McClean Lab
Basics
- Joining the Lab
- Leaving the Lab
- Lab Notebooks
- Dilution of Oligos
- Ordering Media
- Protocol Template
- Lab Database
Introductory Exercises
General Lab Procedures
- Washing Glassware
- Dry Ice-Ethanol Bath
- Pouring Gels for Electrophoresis (Mike)
- Using the Nanodrop2000
Yeast
- Drug Concentrations
- Basic Chemostat Guide
- Bayanus Transformation
- Cleaning Floating Pin Replicators
- Colony PCR (Yeast)
- Fluorescence in situ Hybridization (Colin)
- Fluorescent in situ Hybridization (Ping)
- Fixation of Yeast (Bisaria Protocol)
- Fixation of Yeast (P. Xu Protocol)
- Frogging a Serial Dilution
- Frogging Tetrads
- Genomic DNA Prep (Bust 'n' Grab Protocol)
- GEV Strain Construction
- Glycerol stocks (yeast)
- Hemacytometer protocol for yeast
- Making and Using Frozen Yeast Competant Cells
- Mating Type Testers
- Phluorin Calibration
- Pinning (96 or 384 format)
- Plasmid Loss Assay
- Random Spore Prep
- Sequencing Colony PCR Product
- Smartstat Startup
- Sporulation
- Tetrad Dissection
- URA Pop-out
- Yeast Mating Halo Assay
- Yeast Nomenclature
- Yeast Recombinational Cloning
- Yeast Transformation (S. cerevisiae)
- YMC Induction in Chemostat
- Zygote Picking
- Working with the LoxP/Cre System in S. cerevisiae
- Western Blot
- Membrane Stripping
- Membrane Stripping-Mild
- Invasive growth assay
Bacteria
- Transformation of E. Coli
- E. coli Glycerol Stocks
- E. coli Competent Cells
- Colony PCR (E. coli)
- E. coli Electroporation
- Plasmid Prep from E. coli using a Qiagen Kit
PCR
Flow Cytometry
- General Flow Cytometry Procedure
- Cycloheximide Concentration Assay
- Flourecent Protein Folding Time Assay
- Induction time course
- Phase Plane Mapping
Media
- KS Amino Acid Supplement
- Low Fluorescence Media
- Low Fluorescence Agar Membranes
- Magic Marker Medias
- Quickie YPD supplemented with Adenine
- Synthetic Complete (SC) Yeast Media
- Synthetic Complete (SC) Media w/Drugs
Stock Solutions
- Agarose for gels
- α-Factor 1mg/ml Stock
- Bacterial Glycerol 65%
- Carbenicillin Stock Solution
- Concanavalin A Solution without ions
- Cycloheximide Stock Solution
- DNA Ladder Stock for PCR
- EDTA (0.5M)
- Geneticin/G418 Stock Solution
- PBS/0.1%Tween
- Phosphate Buffered Saline (PBS, 10x)
- Seventy Percent (70%) Ethanol
- TAE Buffer (10X)
- TE Buffer (10X)
- Tris Buffer
Microfluidics
- Concanavalin A Solution for Microfluidics
- Constructing Flow Cells
- FCS2 chamber for Metabolic Cycling
- Bandwidth Experiments
Microscopy
- Microscope Dos and Don'ts
- Nikon TI Basic Use
- Registering objectives on the Nikon
- 96 Well Plate Assay
- NIS Elements Repair
- New Scope Settings
- Quickie ImageJ Quantification
- NikonTI-Eclipse Setup and operation using Micromanager
Misc
- 96 Well Plate Print Out
- AddGene Orders
- Blue Light Overview
- Sequencing
- Bibtex4Word (Add-in for word that allows you to use your existing BibTeX database)
- Orders from IDT/Genewiz/Macrogen/GenScript
- Searching a Sequence for All Database Primers
- Annealing Oligos
- Oligonucleotide phosphorylation, Annealing and Ligation
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