McClean:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(→Yeast) |
(→Yeast) |
||
| Line 18: | Line 18: | ||
*[[McClean: FISH (Ping) | Fluorescent in situ Hybridization (Ping)]] | *[[McClean: FISH (Ping) | Fluorescent in situ Hybridization (Ping)]] | ||
*[[McClean: Frogging_Tetrads | Frogging Tetrads]] | *[[McClean: Frogging_Tetrads | Frogging Tetrads]] | ||
*[[McClean: Genomic DNA Prep (Bust 'n' Grab Protocol)| Genomic DNA Prep (Bust 'n' Grab Protocol)]] | |||
*[[McClean:Mating_Type_Testers | Mating Type Testers]] | *[[McClean:Mating_Type_Testers | Mating Type Testers]] | ||
*[[McClean:Phluorin Calibration | Phluorin Calibration]] | *[[McClean:Phluorin Calibration | Phluorin Calibration]] | ||
Revision as of 08:45, 13 June 2012
Back to McClean Lab
Basics
- Joining the Lab
- Leaving the Lab
- Lab Notebooks
- Dilution of Oligos
- Ordering Media
- Protocol Template
- Lab Database
Yeast
- Basic Chemostat Guide
- Bayanus Transformation
- Cleaning Floating Pin Replicators
- Colony PCR (Yeast)
- Fluorescence in situ Hybridization (Colin)
- Fluorescent in situ Hybridization (Ping)
- Frogging Tetrads
- Genomic DNA Prep (Bust 'n' Grab Protocol)
- Mating Type Testers
- Phluorin Calibration
- Pinning (96 or 384 format)
- Random Spore Prep
- Smartstat Startup
- Sporulation
- Tetrad Dissection
- URA Pop-out
- Yeast Mating Halo Assay
- Yeast Nomenclature
- Yeast Recombinational Cloning
- Yeast Transformation (S. cerevisiae)
- YMC Induction in Chemostat
- Zygote Picking
Bacteria
PCR
Flow Cytometry
- General Flow Cytometry Procedure
- Cycloheximide Concentration Assay
- Flourecent Protein Folding Time Assay
- Induction time course
- Phase Plane Mapping
Media
- KS Amino Acid Supplement
- Low Fluorescence Media
- Low Fluorescence Agar Membranes
- Magic Marker Medias
- Quickie YPD supplemented with Adenine
- Synthetic Complete (SC) Yeast Media
- Synthetic Complete (SC) Media w/Drugs
Stock Solutions
- Agarose for gels
- α-Factor 1mg/ml Stock
- Bacterial Glycerol 65%
- Carbenicillin Stock Solution
- Concanavalin A Solution without ions
- Cycloheximide Stock Solution
- DNA Ladder Stock for PCR
- Geneticin/G418 Stock Solution
- PBS/0.1%Tween
- Phosphate Buffered Saline (PBS, 10x)
- Seventy Percent (70%) Ethanol
Microfluidics
Microscopy
- Microscope Dos and Don'ts
- Nikon TI Basic Use
- 96 Well Plate Assay
- NIS Elements Repair
- New Scope Settings
- Quickie ImageJ Quantification
