McClean:Protocols: Difference between revisions
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*[[McClean: Bacterial Glycerol 65%| Bacterial Glycerol 65%]] | *[[McClean: Bacterial Glycerol 65%| Bacterial Glycerol 65%]] | ||
*[[McClean: PBS/0.1%Tween| PBS/0.1%Tween]] | *[[McClean: PBS/0.1%Tween| PBS/0.1%Tween]] | ||
*[[McClean: Phosphate Buffered Saline (PBS, 10x) | *[[McClean: Phosphate Buffered Saline| Phosphate Buffered Saline (PBS, 10x)]] | ||
==Microfluidics== | ==Microfluidics== | ||
Revision as of 07:22, 6 October 2011
Back to McClean Lab
Basics
Yeast
- Basic Chemostat Guide
- Bayanus Transformation
- Colony PCR (Yeast)
- Fluorescence in situ Hybridization
- Mating Type Testers
- Random Spore Prep
- Sporulation
- Tetrad Dissection
- URA Pop-out
- Yeast Recombinational Cloning
- Yeast Transformation (S. cerevisiae)
- YMC Induction in Chemostat
Bacteria
PCR
Flow Cytometry
- General Flow Cytometry Procedure
- Cycloheximide Concentration Assay
- Flourecent Protein Folding Time Assay
- Induction time course
- Phase Plane Mapping
Media
- Low Fluorescence Media
- Quickie YPD supplemented with Adenine
- Low Fluorescence Agar Membranes
- KS Amino Acid Supplement
Stock Solutions
- Carbenicillin Stock Solution
- DNA Ladder Stock for PCR
- Bacterial Glycerol 65%
- PBS/0.1%Tween
- Phosphate Buffered Saline (PBS, 10x)
