McClean:Oligonucleotide phosphorylation, Annealing and Ligation: Difference between revisions
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(New page: ==Overview== This is a template for how to write a new protocol in openwetware for our lab. In your real protocol, a description of what the protocol is and when to use it would go here. ...) |
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==Overview== | ==Overview== | ||
This is a template for how to write a new protocol in openwetware for our lab. In your real protocol, a description of what the protocol is and when to use it would go here. | This is a template for how to write a new protocol in openwetware for our lab. In your real protocol, a description of what the protocol is and when to use it would go here. | ||
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==Protocol== | ==Protocol== | ||
# Step 1 | |||
# Step 2 | |||
# Step 3 | |||
==Notes== | |||
<!-- Please paste this section "as is" into your protocol, and add notes to it if you have some!--> | |||
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input! | |||
#List troubleshooting tips here. | |||
#You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites. | |||
#Anecdotal observations that might be of use to others can also be posted here. | |||
Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip. | |||
==References== | |||
Gietz, R.D. and R.A. Woods. (2002) TRANSFORMATION OF YEAST BY THE Liac/SS | |||
CARRIER DNA/PEG METHOD. Methods in Enzymology 350: 87-96. | |||
==Contact== | |||
<!--Change the information below to your info if you add a new protocol--> | |||
*'''[[User:Megan N McClean|Megan N McClean]] 14:01, 20 July 2011 (EDT)''' | |||
or instead, [[Talk:{{PAGENAME}}|discuss this protocol]]. | |||
<!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. --> | |||
[[Category:Protocol]] | |||
<!-- Move the relevant categories above this line to tag your protocol with the label | |||
[[Category:Protocol]] | |||
[[Category:Media]] | |||
[[Category:Yeast]] | |||
--> | |||
Revision as of 08:08, 24 October 2013
Overview
This is a template for how to write a new protocol in openwetware for our lab. In your real protocol, a description of what the protocol is and when to use it would go here.
Materials
- Item 1
- Item 2
- Stock Solution 1
- Stock Solution 2
Stock Solutions
Stock Solution 1
- This is a very simple solution, so we only need a one line description of how to make it.
Stock Solution 2
This is a more involved solution, so we will describe how to make it in several steps:
- Step 1
- Step 2
- Step 3
Protocol
- Step 1
- Step 2
- Step 3
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
References
Gietz, R.D. and R.A. Woods. (2002) TRANSFORMATION OF YEAST BY THE Liac/SS CARRIER DNA/PEG METHOD. Methods in Enzymology 350: 87-96.
Contact
- Megan N McClean 14:01, 20 July 2011 (EDT)
or instead, discuss this protocol.
