McClean:Magic Marker Medias

Overview

Medias used for doing strain construction with the magic marker technology (see references)

10x SD/MSG

• 1.7 g YNB without amino acids without ammonium sulfate (Difco, #233520)
• 1 g Monosodium glutamate (L-glutamic acid, monosodium salt MP BIomedicals #194677)

Combine YNB and monosodium glutamate in 70mls of dH₂O. Dissolve the glutamate and YNB and then bring the final volume to 100mls. Autoclave. This solution is 10x.