McClean:Magic Marker Medias: Difference between revisions
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(New page: ==Overview== Medias used for doing strain construction with the magic marker technology (see references) ==10x SD/MSG== 1.7 g YNB without amino acids without ammonium sulfate (Difco, #23...) |
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==Overview== | ==Overview== | ||
Medias used for doing strain construction with the magic marker technology (see references) | Medias used for doing strain construction with the magic marker technology (see references) | ||
==10x SD/MSG== | ==10x SD/MSG== | ||
1.7 g YNB without amino acids without ammonium sulfate (Difco, #233520) | * 1.7 g YNB without amino acids without ammonium sulfate (Difco, #233520) | ||
1 g Monosodium glutamate (L-glutamic acid, monosodium salt MP BIomedicals #194677) | *1 g Monosodium glutamate (L-glutamic acid, monosodium salt MP BIomedicals #194677) | ||
Combine YNB and monosodium glutamate in 70mls of dH<sub>2</sub>O. Dissolve the glutamate and YNB and then bring the final volume to 100mls. Autoclave. This solution is 10x. | Combine YNB and monosodium glutamate in 70mls of dH<sub>2</sub>O. Dissolve the glutamate and YNB and then bring the final volume to 100mls. Autoclave. This solution is 10x. | ||
Revision as of 12:19, 24 January 2012
Overview
Medias used for doing strain construction with the magic marker technology (see references)
10x SD/MSG
- 1.7 g YNB without amino acids without ammonium sulfate (Difco, #233520)
- 1 g Monosodium glutamate (L-glutamic acid, monosodium salt MP BIomedicals #194677)
Combine YNB and monosodium glutamate in 70mls of dH2O. Dissolve the glutamate and YNB and then bring the final volume to 100mls. Autoclave. This solution is 10x.
