McClean:Magic Marker Medias

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(New page: ==Overview== Medias used for doing strain construction with the magic marker technology (see references) ==10x SD/MSG== 1.7 g YNB without amino acids without ammonium sulfate (Difco, #23...)
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==Overview==
==Overview==
Medias used for doing strain construction with the magic marker technology (see references)
Medias used for doing strain construction with the magic marker technology (see references)
 +
==10x SD/MSG==
==10x SD/MSG==
-
1.7 g YNB without amino acids without ammonium sulfate (Difco, #233520)
+
* 1.7 g YNB without amino acids without ammonium sulfate (Difco, #233520)
-
1 g Monosodium glutamate (L-glutamic acid, monosodium salt MP BIomedicals #194677)
+
*1 g Monosodium glutamate (L-glutamic acid, monosodium salt MP BIomedicals #194677)
Combine YNB and monosodium glutamate in 70mls of dH<sub>2</sub>O.  Dissolve the glutamate and YNB and then bring the final volume to 100mls.  Autoclave.  This solution is 10x.
Combine YNB and monosodium glutamate in 70mls of dH<sub>2</sub>O.  Dissolve the glutamate and YNB and then bring the final volume to 100mls.  Autoclave.  This solution is 10x.

Revision as of 15:19, 24 January 2012

Overview

Medias used for doing strain construction with the magic marker technology (see references)


10x SD/MSG

  • 1.7 g YNB without amino acids without ammonium sulfate (Difco, #233520)
  • 1 g Monosodium glutamate (L-glutamic acid, monosodium salt MP BIomedicals #194677)

Combine YNB and monosodium glutamate in 70mls of dH2O. Dissolve the glutamate and YNB and then bring the final volume to 100mls. Autoclave. This solution is 10x.

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