McClean:LB1: Difference between revisions

Revision as of 10:24, 2 July 2015

Liquid LB media

Begin with approximately 800mls of MiliQ water
Weigh out and add ingredients with constant stirring:
- Bacto tryptone 10g
- Bacto yeast extract 5g
- NaCl 5g
- Bacto Agar 15g

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

List troubleshooting tips here.
You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

*Megan N McClean 13:24, 2 July 2015 (EDT) Many LB formulations call for twice as much NaCl (10g instead of 5g in this protocol).

Revision as of 10:24, 2 July 2015 (view source) Megan N McClean (talk \| contribs) (New page: ==Overview== Liquid LB media ==Reagents== * Bacto tryptone * Bacto yeast extract * NaCl * Bacto Agar ==Protocol== * Begin with approximately 800mls of MiliQ water * Weigh out and add ing...)		Revision as of 10:24, 2 July 2015 (view source) Megan N McClean (talk \| contribs) (→‎Notes) Newer edit →
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	Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.		Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.


	'''*[[User:Megan N McClean\|Megan N McClean]] 13:24, 2 July 2015 (EDT)''' Many LB formulations call for twice as much NaCl (10g instead of 5g in this protocol).		'''*[[User:Megan N McClean\|Megan N McClean]] 13:24, 2 July 2015 (EDT)''' Many LB formulations call for twice as much NaCl (10g instead of 5g in this protocol).