McClean:LB1: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(→Notes) |
No edit summary |
||
| (One intermediate revision by one other user not shown) | |||
| Line 1: | Line 1: | ||
==Overview== | ==Overview== | ||
LB agar media | |||
==Reagents== | ==Reagents== | ||
| Line 16: | Line 16: | ||
** Bacto Agar 15g | ** Bacto Agar 15g | ||
*Bring up to | *Bring up to 1000mls with MiliQ water | ||
*Separate into two 1L bottoles of 500mls each | *Separate into two 1L bottoles of 500mls each | ||
*Autoclave | *Autoclave | ||
Latest revision as of 13:12, 26 August 2015
Overview
LB agar media
Reagents
- Bacto tryptone
- Bacto yeast extract
- NaCl
- Bacto Agar
Protocol
- Begin with approximately 800mls of MiliQ water
- Weigh out and add ingredients with constant stirring:
- Bacto tryptone 10g
- Bacto yeast extract 5g
- NaCl 5g
- Bacto Agar 15g
- Bring up to 1000mls with MiliQ water
- Separate into two 1L bottoles of 500mls each
- Autoclave
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
*Megan N McClean 13:24, 2 July 2015 (EDT) Many LB formulations call for twice as much NaCl (10g instead of 5g in this protocol).
References
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
