McClean:KS Amino Acid Supplement

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Contents

Summary

This is the amino acid supplement used in our low fluorescence media and synthetic complete medias. To make dropout media, leave out the appropriate amino acid (ie, to make SC-URA, leave out uracil). These supplements are stored as dry powder stocks on the media shelf in brown bottles (some amino acids are light sensitive). If you need to remake a supplement stock, please use exactly the amino acids listed (take note of catalog numbers) and clearly initial and date the bottle.

Final Composition in Liquid Media

Amino Acid Supplier Description Catalog No. KS Supp Final (mg/L) KS Supp 10x stock (mg/L)
adenine hemisulfateSigmaAdnine hemisulfate salt, min 99%A912650500
uracilMP BiomedicalsUracil10320420200
tryptophanFisherL-tryptophan, tissue culture gradeBP39550500
histidine-HClFisherL-histiine, mono HCL monohydrateBP38320200
arginine-HClFisherL-arginine HCLBP37220200
methionineFisherL-methionineBP38820200
tyrosineFisherL-tyrosineBP39630300
leucineFisherL-leucineBP3851001000
isoleucineFisherL-isoleucineBP38430300
lysine-HClFisherL-lysine monohydrochlorideBP3861001000
phenylalanineFisherL-phenylalanineBP39150500
glutamic acidFisherL-glutamic acid monosodium salt1946771001000
aspartic acid FisherL-aspartic acidBP3741001000
valineFishervalineBP3971501500
threonineFisherL-threonineBP3942002000
serineAcros OrganicsL-serine 99%1326610004004000

Dry Powder Stocks

To make dry stocks mix all items together (leave out specific amino acids if desired) in a brown plastic bottle, turn end over end MANY MANY times to mix with a sterile marble added to aid mixing. Sterile marbles are stored on the media shelf. Store the final mix dry on the media shelf in a brown opaque bottle. Initial and clearly date your new amino acid stock.

Amino Acid Supplier Description Catalog No. Dry power enough for 10-1L 10x stocks (mg) Dry power enough for 10-1L 10x stocks (grams) Dry power enough for 5-1L 10x stocks (grams)
adenine hemisulfateSigmaAdnine hemisulfate salt, min 99%A9126500052.5
uracilMP BiomedicalsUracil103204200021
tryptophanFisherL-tryptophan, tissue culture gradeBP395500052.5
histidine-HClFisherL-histiine, mono HCL monohydrateBP383200021
arginine-HClFisherL-arginine HCLBP372200021
methionineFisherL-methionineBP388200021
tyrosineFisherL-tyrosineBP396300031.5
leucineFisherL-leucineBP38510000105
isoleucineFisherL-isoleucineBP384300031.5
lysine-HClFisherL-lysine monohydrochlorideBP38610000105
phenylalanineFisherL-phenylalanineBP391500052.5
glutamic acidFisherL-glutamic acid monosodium salt19467710000105
aspartic acid FisherL-aspartic acidBP37410000105
valineFishervalineBP39715000157.5
threonineFisherL-threonineBP394200002010
serineAcros OrganicsL-serine 99%132661000400004020

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

This amino acid mix was developed by Katja Schwartz (KS) in the Botstein lab at Stanford University. It is adapted from the synthetic complete dropout mix in the CSH Yeast Genetics Course lab manual.

Contact

or instead, discuss this protocol.


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