McClean:Invasive growth assay

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(New page: ==Overview== This is a template for how to do yeast invasive growth assay. ==Protocol== # Make YPD plates with 1M Sobitol. # Grow strains to saturate. # Do serial dilution in 96 well pla...)
(Notes)
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Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
#If you can't get good pictures, you can grow your rubbed plate for couple days, take picture again. Or you can grow your plate for more days before you wash it.   
#If you can't get good pictures, you can grow your rubbed plate for couple days, take picture again. Or you can grow your plate for more days before you wash it.   
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#You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
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#Anecdotal observations that might be of use to others can also be posted here. 
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Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.
Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.

Revision as of 14:43, 8 November 2013

Contents

Overview

This is a template for how to do yeast invasive growth assay.


Protocol

  1. Make YPD plates with 1M Sobitol.
  2. Grow strains to saturate.
  3. Do serial dilution in 96 well plate.
  4. Frog to 1M Sobitol YPD plate.
  5. Grow the plate in 30 degree for 2-3 days, then rub the plate under steam of deionized water.
  6. Proceed to take picture.


Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. If you can't get good pictures, you can grow your rubbed plate for couple days, take picture again. Or you can grow your plate for more days before you wash it.


Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

Contact

or instead, discuss this protocol.

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