McClean:Drug Concentrations: Difference between revisions

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(New page: Yeast Media Antibiotic Concentrations • G418: o Stock is 200 mg/mL (1000X) o Final concentration 200 ug/ml o Use 1ul stock per ml of media • ClonNAT: o Stock is 50 mg/ml (1000X) o F...)
 
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Yeast Media Antibiotic Concentrations
Yeast Media Antibiotic Concentrations
• G418:  
*G418:  
o Stock is 200 mg/mL (1000X)
**Stock is 200 mg/mL (1000X)
o Final concentration 200 ug/ml   
**Final concentration 200 ug/ml   
o Use 1ul stock per ml of media
**Use 1ul stock per ml of media
• ClonNAT:
*ClonNAT:
o Stock is 50 mg/ml (1000X)
**Stock is 50 mg/ml (1000X)
o Final concentration 50 ug/mL  
**Final concentration 50 ug/mL  
o Use 1 ul stock per ml of media
**Use 1 ul stock per ml of media
• HygromycinB:
*HygromycinB:
o Stock is 50 mg/mL (166X)
**Stock is 50 mg/mL (166X)
o Final concentration 300 ug/mL
**Final concentration 300 ug/mL
o Use 6ul of stock per ml of media
**Use 6ul of stock per ml of media

Latest revision as of 09:58, 20 March 2016

Yeast Media Antibiotic Concentrations

  • G418:
    • Stock is 200 mg/mL (1000X)
    • Final concentration 200 ug/ml
    • Use 1ul stock per ml of media
  • ClonNAT:
    • Stock is 50 mg/ml (1000X)
    • Final concentration 50 ug/mL
    • Use 1 ul stock per ml of media
  • HygromycinB:
    • Stock is 50 mg/mL (166X)
    • Final concentration 300 ug/mL
    • Use 6ul of stock per ml of media
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