Matthew E. Jurek Week 5

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(Saccharomyces Genome Database: fixed all the links)
(Saccharomyces Genome Database: fixed GDH2 link)
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**''GDH1''- Expression is regulated by carbon and nitrogen sources. [http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=GDH1 GDH1]
**''GDH1''- Expression is regulated by carbon and nitrogen sources. [http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=GDH1 GDH1]
**''GDH3''- Expression is regulated by carbon and nitrogen sources. [http://www.yeastgenome.org/cgi-bin/locus.fpl?dbid=S000000058 GDH3]
**''GDH3''- Expression is regulated by carbon and nitrogen sources. [http://www.yeastgenome.org/cgi-bin/locus.fpl?dbid=S000000058 GDH3]
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**''GDH2''- Expression is regulated by nitrogen catabolite repression and intracellular ammonia levels. [http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=GDH2 "GDH2']
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**''GDH2''- Expression is regulated by nitrogen catabolite repression and intracellular ammonia levels. [http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=GDH2 GDH2]
**''GLN1''- Expression is regulated by nitrogen source and by amino acid limitation. [http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=GLN1 GLN1]
**''GLN1''- Expression is regulated by nitrogen source and by amino acid limitation. [http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=GLN1 GLN1]
**''GLT1'' - Expression is regulated by nitrogen source. [http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=GLT1 GLT1]
**''GLT1'' - Expression is regulated by nitrogen source. [http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=GLT1 GLT1]

Revision as of 14:37, 14 February 2013

Matthew E. Jurek BIOL398-03/S13

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Saccharomyces Genome Database

  • Which of these genes has a homolog (similar gene related by descent) in humans? What disease does a deficiency of this gene cause in humans?
    • GDH2 has the following homologs in humans: GLUD1 and GLUD2. Deficiencies in these result in hyperinsulinism-hyperammonemia syndrome as well as other neurological disorders. GDH2
  • How is the expression of each of these genes regulated?
    • GDH1- Expression is regulated by carbon and nitrogen sources. GDH1
    • GDH3- Expression is regulated by carbon and nitrogen sources. GDH3
    • GDH2- Expression is regulated by nitrogen catabolite repression and intracellular ammonia levels. GDH2
    • GLN1- Expression is regulated by nitrogen source and by amino acid limitation. GLN1
    • GLT1 - Expression is regulated by nitrogen source. GLT1
  • Using the compound search tool of SGD, search on "L-glutamate". How many pathways does it participate in?
  • Find the SGD representation of the pathway we are working on in class and attach a screenshot and hyperlink to your journal page. Choose the one that shows all of the reactions we talked about in class and make sure you can relate it to your notes, matching the genes, enzyme names, and reactants/products.
  • What parameters for these reactions can you find using this database? HINT: the literature portion of the individual gene pages may be helpful.
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