Matthew E. Jurek Week 3

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===Outline===
===Outline===
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====Introduction====
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*Ammonia is the preferred nitrogen source of yeast,''Saccharomyces cerevisiae'', as noted by rapid growth.
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*The breakdown, or metabolism, of nitrogen is regulated at two levels: gene expression and enzyme activity.
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*Continuous cultures differ in external ammonia concentration and ammonia assimilation.
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*It is thought the flux of ammonia is more critical in nitrogen breakdown than ammonia concentration.

Revision as of 22:54, 30 January 2013

Matthew E. Jurek BIOL398-03/S13

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The Concentration of Ammonia Regulates Nitrogen Metabolism in Saccharomyces cerevisiae

Eelko G. ter Shure, Herman H.W. Sillje, Arie J. Verkleij, Johannes Boonstra, and C. Theo Verrips

Defining Terms

All definitions from biology-online.org [1]

  • Biosynthetic- Relating to or produced by biosynthesis.
  • Flux-(Science: radiobiology) The total amount of a quantity passing through a given surface per unit time. Typical quantities include (magnetic) field lines, particles, heat, energy, mass of fluid, etc. Common usage in plasma physics is for flux by itself to mean magnetic field flux, unless specified otherwise.
  • Glutamate-(Science: biochemistry, physiology) major fast excitatory neurotransmitter in the mammalian central nervous system.
  • Metabolite-(Science: biochemistry) Any substance produced by metabolism or by a metabolic process. Any substance involved in metabolism (either as a product of metabolism or as necessary for metabolism). An end product as a result of metabolism.
  • Proline- (Science: amino acid) One of the 20 amino acids directly coded for in proteins. Structure differs from all the others, in that its side chain is bonded to the nitrogen of the amino group, as well as the carbon. This makes the amino group a secondary amine and so proline is described as an imino acid. Has strong influence on secondary structure of proteins and is much more abundant in collagens than in other proteins, occurring especially in the sequence glycine proline hydroxyproline. A proline rich region seems to characterise the binding site of SH3 domains. An amino acid that is found in many proteins (especially collagen).One of 20 amino acids commonly found as part of a protein.

Outline

Introduction

  • Ammonia is the preferred nitrogen source of yeast,Saccharomyces cerevisiae, as noted by rapid growth.
  • The breakdown, or metabolism, of nitrogen is regulated at two levels: gene expression and enzyme activity.
  • Continuous cultures differ in external ammonia concentration and ammonia assimilation.
  • It is thought the flux of ammonia is more critical in nitrogen breakdown than ammonia concentration.
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