Matthew E. Jurek Week 14

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(Electronic Laboratory Notebook: documented part 3)
(Electronic Laboratory Notebook: documented part 4)
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#.My network consists of 23 transcription factors based on the Week 12 Assignment. The Regulation Matrix has been copied onto the 3 appropriate worksheets: network, network_weights, and network_thresholds. The transcription factors are not in alphabetical order, rather I will keep them in the order generated by Yeastract, ensuring each worksheet utilizes the same order. Following the important step in, HMO1 was deleted from my network as that is the gene I was assigned. My worksheets have been adjusted accordingly.
#.My network consists of 23 transcription factors based on the Week 12 Assignment. The Regulation Matrix has been copied onto the 3 appropriate worksheets: network, network_weights, and network_thresholds. The transcription factors are not in alphabetical order, rather I will keep them in the order generated by Yeastract, ensuring each worksheet utilizes the same order. Following the important step in, HMO1 was deleted from my network as that is the gene I was assigned. My worksheets have been adjusted accordingly.
#The standard names have been copied and pasted into Yeastract. I have now received the systematic name and have placed them in the appropriate column. The degradation rates are being explored according to the Belle et al. data. Only about 4 transcription factors are not on this list, thus warranting a rate of .027182242.
#The standard names have been copied and pasted into Yeastract. I have now received the systematic name and have placed them in the appropriate column. The degradation rates are being explored according to the Belle et al. data. Only about 4 transcription factors are not on this list, thus warranting a rate of .027182242.
 +
#Production rates have been found by creating a column next to the degradation rates. A formula has been applied that multiplies these values by 2. With the degradation rates doubled, I now have the production rates. These will be copied and pasted on the production_rates worksheet.

Revision as of 21:05, 25 April 2013

Matthew E. Jurek BIOL398-03/S13

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Electronic Laboratory Notebook

  1. . Began by opening the sample data and renaming it to Jurek_Week14 before beginning manipulations.
  2. .My network consists of 23 transcription factors based on the Week 12 Assignment. The Regulation Matrix has been copied onto the 3 appropriate worksheets: network, network_weights, and network_thresholds. The transcription factors are not in alphabetical order, rather I will keep them in the order generated by Yeastract, ensuring each worksheet utilizes the same order. Following the important step in, HMO1 was deleted from my network as that is the gene I was assigned. My worksheets have been adjusted accordingly.
  3. The standard names have been copied and pasted into Yeastract. I have now received the systematic name and have placed them in the appropriate column. The degradation rates are being explored according to the Belle et al. data. Only about 4 transcription factors are not on this list, thus warranting a rate of .027182242.
  4. Production rates have been found by creating a column next to the degradation rates. A formula has been applied that multiplies these values by 2. With the degradation rates doubled, I now have the production rates. These will be copied and pasted on the production_rates worksheet.
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