Matthew E. Jurek Week 12: Difference between revisions

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#*Cytoplasm-All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
#*Cytoplasm-All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
#*Cytosol-The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
#*Cytosol-The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
#*Response on stress-
#*Response on stress-Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a disturbance in organismal or cellular homeostasis, usually, but not necessarily, exogenous (e.g. temperature, humidity, ionizing radiation).
#*Regulation of phosphate metabolic process-Any process that modulates the frequency, rate or extent of the chemical reactions and pathways involving phosphates.
#*Regulation of phosphate metabolic process-Any process that modulates the frequency, rate or extent of the chemical reactions and pathways involving phosphates.
#*Protein Binding-Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
#*Protein Binding-Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).

Revision as of 15:05, 10 April 2013

Matthew E. Jurek BIOL398-03/S13

Assignment Page

User Page

Analyzing and Interpreting STEM Results

  1. Why did you select this profile? In other words, why was it interesting to you?
    • I chose Profile 9 because it is down-regulated. My partner, Anthony, is focusing on a profile that is up-regulated. We thought it'd be interesting if we each explored a profile that behaved differently.
  2. How many genes belong to this profile?
    • There are 95 genes belonging to this profile.
  3. How many genes were expected to belong to this profile?
    • 23.9 genes were expected to belong to this profile.
  4. What is the p value for the enrichment of genes in this profile?
    • The p-value for this profile is 3.3X10-29.
  5. How many GO terms are associated with this profile at p < 0.05?
    • 14 GO terms are associated with this p-value.
  6. How many GO terms are associated with this profile with a corrected p value < 0.05?
    • 1 GO term is associated with this corrected p-value.
  7. Look up the definitions for each of the terms at http://geneontology.org. Write a paragraph that describes the biological interpretation of these GO terms. In other words, why does the cell react to cold shock by changing the expression of genes associated with these GO terms?
    • Protein folding-The process of assisting in the covalent and noncovalent assembly of single chain polypeptides or multisubunit complexes into the correct tertiary structure.
    • Unfolded protein binding-Interacting selectively and non-covalently with an unfolded protein.
    • Cytoplasm-All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
    • Cytosol-The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
    • Response on stress-Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a disturbance in organismal or cellular homeostasis, usually, but not necessarily, exogenous (e.g. temperature, humidity, ionizing radiation).
    • Regulation of phosphate metabolic process-Any process that modulates the frequency, rate or extent of the chemical reactions and pathways involving phosphates.
    • Protein Binding-Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
    • Regulation of biological quality-Any process that modulates a qualitative or quantitative trait of a biological quality. A biological quality is a measurable attribute of an organism or part of an organism, such as size, mass, shape, color, etc.
    • Carboxylic acid catabolic process-The chemical reactions and pathways resulting in the breakdown of carboxylic acids, any organic acid containing one or more carboxyl (-COOH) groups.
    • Small molecule metabolic catabolic process-The chemical reactions and pathways resulting in the breakdown of small molecules, any low molecular weight, monomeric, non-encoded molecule.