Matthew E. Jurek Week 11

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#transcriptome- the transcriptome is the set of all RNA molecules, including mRNA, rRNA, tRNA, and non-coding RNA produced in one or a population of cells.
#transcriptome- the transcriptome is the set of all RNA molecules, including mRNA, rRNA, tRNA, and non-coding RNA produced in one or a population of cells.
via [http://www.biology-online.org/dictionary/Main_Page| Biology Online Dictionary]
via [http://www.biology-online.org/dictionary/Main_Page| Biology Online Dictionary]
 +
==Acclimation of ''Saccharomyces cerevisiae'' to Low Temperature: A Chemostat-based Transcriptome Analyis==
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Tai et al. (2007) Acclimation of Saccharomyces cerevisiae to Low Temperature: A Chemostat-based Transcriptome Analysis. Molecular Biology of the Cell 18: 5100–5112.
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#Introduction
 +
*On the cellular level, yeast responds in a number of ways to temperature changes (temps outside the 25-30 degree C optimum).
 +
*Temperatures below the optimum range slow cellular processes.
 +
*Effects of low temperature depend on exposure time
 +
*Sudden exposure results in adaptaion
 +
*Prolonged exposure results in acclimation
 +
*Previous studies have focused on cold shock (sudden exposure) and found 2 phases of cold-shock response
 +
*Current low-temp transcriptome databases contain major discrepancies
 +
*Previous studies have focused on batch culutures: hard to distinguish temp effects on transcription from effects of specific growth rate
 +
*Chemostat cultures allow for control of specific growth rate independent of culture conditions such as metabolites, pH, and oxygen availability.
 +
*Overall Goal: Focus on genome-wide transcriptional regulation by exploring steady-state acclimatized growth of yeast at low temps

Revision as of 00:14, 4 April 2013

Matthew E. Jurek BIOL398-03/S13

Contents

Assignment Page

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Biological Terms

  1. trehalose- also known as mycose or tremalose, is a natural alpha-linked disaccharide formed by an α,α-1,1-glucoside bond between two α-glucose units.
  2. mannoprotein- component of yeast cell walls; protein covalently linked to polymers of mannose.
  3. chromatography- the separation of mixtures into their constituents by preferential adsorption by a solid, as a column of silica (column chromatography) or a strip of filter paper (paper chromatography) or by a gel.
  4. immunoprecipitation- the separation of an antigen from a solution by the formation of a large complex with its specific antibody.
  5. catabolite- a product of catabolic action.
  6. kinetics- the branch of mechanics that deals with the actions of forces in producing or changing the motion of masses.
  7. ceramides- ceramides are a family of lipid molecules. A ceramide is composed of sphingosine and a fatty acid.
  8. orthologue- one of two or more homologous gene sequences found in different species.
  9. ergosterol- a compound present in ergot and many other fungi. A steroid alcohol, it is converted to vitamin D2 when irradiated with ultraviolet light.
  10. transcriptome- the transcriptome is the set of all RNA molecules, including mRNA, rRNA, tRNA, and non-coding RNA produced in one or a population of cells.

via Biology Online Dictionary

Acclimation of Saccharomyces cerevisiae to Low Temperature: A Chemostat-based Transcriptome Analyis

Tai et al. (2007) Acclimation of Saccharomyces cerevisiae to Low Temperature: A Chemostat-based Transcriptome Analysis. Molecular Biology of the Cell 18: 5100–5112.

  1. Introduction
  • On the cellular level, yeast responds in a number of ways to temperature changes (temps outside the 25-30 degree C optimum).
  • Temperatures below the optimum range slow cellular processes.
  • Effects of low temperature depend on exposure time
  • Sudden exposure results in adaptaion
  • Prolonged exposure results in acclimation
  • Previous studies have focused on cold shock (sudden exposure) and found 2 phases of cold-shock response
  • Current low-temp transcriptome databases contain major discrepancies
  • Previous studies have focused on batch culutures: hard to distinguish temp effects on transcription from effects of specific growth rate
  • Chemostat cultures allow for control of specific growth rate independent of culture conditions such as metabolites, pH, and oxygen availability.
  • Overall Goal: Focus on genome-wide transcriptional regulation by exploring steady-state acclimatized growth of yeast at low temps
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